At last some honest research and testing of ionic silver. Note that electrically produced ionic silver outperforms silver compounds by a wide margin.
http://www.ncbi.nlm.nih.gov/pmc/arti...report=classic <http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2292600/?report=classic> Antibacterial Activity and Mechanism of Action of the Silver Ion in Staphylococcus aureus and Escherichia coli. The antibacterial effect and mechanism of action of a silver ion solution that was electrically generated were investigated for Staphylococcus aureus and Escherichia coli by analyzing the growth, morphology, and ultrastructure of the bacterial cells following treatment with the silver ion solution. Bacteria were exposed to the silver ion solution for various lengths of time, and the antibacterial effect of the solution was tested using the conventional plate count method and flow cytometric (FC) analysis. Reductions of more than 5 log10 CFU/ml of both S. aureus and E. coli bacteria were confirmed after 90 min of treatment with the silver ion solution. Significant reduction of S. aureus and E. coli cells was also observed by FC analysis; however, the reduction rate determined by FC analysis was less than that determined by the conventional plate count method. These differences may be attributed to the presence of bacteria in an active but nonculturable (ABNC) state after treatment with the silver ion solution. Transmission electron microscopy showed considerable changes in the bacterial cell membranes upon silver ion treatment, which might be the cause or consequence of cell death. In conclusion, the results of the present study suggest that silver ions may cause S. aureus and E. coli bacteria to reach an ABNC state and eventually die. Since ancient times, the silver ion has been known to be effective against a broad range of microorganisms. Today, silver ions are used to control bacterial growth in a variety of medical applications, including dental work, catheters, and the healing of burn wounds (17, 30, 31). Silver ions are also used for a number of nonmedical purposes, such as in electrical appliances (14, 36). The slow-release “nanosilver” linings of laundry machines, dishwashers, refrigerators, and toilet seats are also marketed and advertised. It is clear that we are exposed to a wide range of mostly unfamiliar uses of silver-containing products intended to function as antimicrobial biocides. Therefore, it is necessary to elucidate the antimicrobial activity of the silver ion, which is widely used in these products. The mechanism of the antimicrobial action of silver ions is closely related to their interaction with thiol (sulfhydryl) groups (1, 5, 9, 10), although other target sites remain a possibility (27, 34). Amino acids, such as cysteine, and other compounds containing thiol groups, such as sodium thioglycolate, neutralized the activity of silver against bacteria (18). By contrast, disulfide bond-containing amino acids, non-sulfur-containing amino acids, and sulfur-containing compounds, such as cystathione, cysteic acid, l-methionine, taurine, sodium bisulfate, and sodium thiosulfate, were all unable to neutralize the activity of silver ions. These and other findings imply that the interaction of silver ions with thiol groups in enzymes and proteins plays an essential role in its antimicrobial action, although other cellular components, like hydrogen bonding, may also be involved (10). Silver was also proposed to act by binding to key functional groups of enzymes. Silver ions cause the release of K+ ions from bacteria; thus, the bacterial plasma or cytoplasmic membrane, which is associated with many important enzymes, is an important target site for silver ions (9, 22, 25, 29). In addition to their effects on bacterial enzymes, silver ions caused marked inhibition of bacterial growth and were deposited in the vacuole and cell wall as granules (6). They inhibited cell division and damaged the cell envelope and contents of bacteria (27). Bacterial cells increased in size, and the cytoplasmic membrane, cytoplasmic contents, and outer cell layers all exhibited structural abnormalities. Finally, silver ions interact with nucleic acids (35); they interact preferentially with the bases in DNA rather than with the phosphate groups, although the significance of this in terms of their lethal action is unclear (12, 24, 34, 37). The following silver compounds and silver are listed in Martindale: the Extra Pharmacopoeia: silver metal, silver acetate, silver nitrate, silver protein, and silver sulfadiazine (26a). The silver ion can be generated by electrolyzing the silver metal or dissolving the silver compounds. It is known that the electrically generated silver ion appeared to be superior to the silver compounds in antimicrobial activity (3, 4). However, most of the aforementioned studies which determined a mechanism of action of silver used silver ions produced from silver compounds like silver nitrate or silver sulfadiazine, and thus there has been limited research on the electrically generated silver ion. Recently, a laundry machine that emits electrically generated silver ions was developed for hygiene, namely, in order to prevent easily transmissible bacterial and fungal skin infections from being transmitted by contaminated laundry. In particular, it can be beneficial to hospitals and homes in which immunocompromised people (the elderly, children, and medical patients) or pets may dwell. Our previous study demonstrated the antifungal activity of a laundry machine that electrically generates silver ions (14). In the present study, we used conventional plate counting, flow cytometry (FC), and transmission electron microscopy (TEM) to investigate the antibacterial activity and mechanism of action against Staphylococcus aureus and Escherichia coli bacteria of a silver ion solution generated from the laundry machine.It is known that the electrically generated silver ion appeared to be superior to the silver compounds in antimicrobial activity.
