Hydrogen peroxide notes Date: Tue, 10 Oct 2000 17:20:32 -0700 From: "Dr. Carolyn Bormann" <[email protected] Subject: Oxylist Peroxide, kidney damage, NAC
Dear List: Thought since many on the list use H202 (peroxide) orally that I would pass this along. I've always taught my patients to NEVER drink diluted peroxide water (despite Ed's book et al on the popular Urban Trends, etc.) because I knew it scarred kidney tubules (I have had 2 friends damaged, one so badly she lost 1 kidney). It is a matter of TISSUE antioxidant levels (not blood), and that is a trickey thing that is dependent on many factors such as circulation, other things that can compete for the molecules, the other oxidative challenges going on, etc. We've used NAC for about 6 years clinically for patients automatically knowing it helps with excess free radical damage via the pyruvate pathway (we use alot of ozone and uvbi). Hope it's helpful... Dr. Bormann arrowheadhealthworks.com http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMe d&list_uids=10851302&dopt=Abstract : Mol Urol 2000 Spring;4(1):15-20 TITLE: Adverse effects of oxidative stress on renal cells and its prevention by antioxidants. Nowzari FB, Davidson SD, Eshghi M, Mallouh C, Tazaki H, Konno S Department of Urology, New York Medical College, Valhalla, New York. [Medline record in process] Background and Purpose: Recent reports suggest that reactive oxygen species; e.g., hydrogen peroxide (H(2)O(2)), could be the primary cause of various drug-induced renal injuries. We investigated the effects of H(2)O(2) on renal cells to understand its mode of action and to explore cytoprotection from such a fatal injury. Materials and Methods: Renal proximal tubular LLC-PK(1) cells were exposed to various concentrations of H(2)O(2), and cell viability was determined at specified times. Lipid peroxidation assay and Western blot analysis of heat shock proteins (Hsp70 and Hsp90) were performed to assess the cellular effects. Results: The dose-response study showed that H(2)O(2) /= 100 &mgr;M was severely cytotoxic. Even a 1-h exposure was sufficient to induce 95% cell death in 24 h. Lipid peroxidation was significantly (50%) increased, while Hsp90, but not Hsp70, was partially degraded, to an approximately 85-kDa fragment, after a 3-h H(2)O(2) exposure. However, such cytotoxic cell death was remarkably ( approximately 90%) prevented by the antioxidants pyruvate or N-acetylcysteine (NAC), and Hsp90 remained intact. Conclusion: Hydrogen peroxide-induced renal cell death involves increased lipid peroxidation and partial degradation of Hsp90. Both pyruvate and NAC are capable of detoxifying H(2)O(2) to maintain cell viability and Hsp90 integrity. Acute renal injuries associated with oxidative stress might preventable by appropriate antioxidants. -- The silver-list is a moderated forum for discussion of colloidal silver. To join or quit silver-list or silver-digest send an e-mail message to: [email protected] -or- [email protected] with the word subscribe or unsubscribe in the SUBJECT line. To post, address your message to: [email protected] Silver-list archive: http://escribe.com/health/thesilverlist/index.html List maintainer: Mike Devour <[email protected]>
