Ok. Thanks for the help. I'll let you know what I find during the
analyses.
-Rebekah

On Jan 29, 1:42 pm, Luis Mendoza <[email protected]> wrote:
> Hello Rebekah,
> I have not heard of anyone using Libra on TMT data, but it should be able to
> handle it.
>
> You will first need to generate a Libra condition file, where you specify
> some options as well as isotopic contributions to each channel.  (more info
> here:  http://tools.proteomecenter.org/wiki/index.php?title=Software:Libra
> and 
> here:http://sashimi.svn.sourceforge.net/viewvc/sashimi/trunk/trans_proteom...).
>  I am not familiar with the Sorcerer interface, but recent releases of
> the TPP GUI have a screen where you can generate this file (under Utilities
> -> Libra Conditions). The interface has a drop-down that will pre-populate
> the channel masses, though you will nee to enter the isotopic contributions
> to neighboring channels (or leave them at zero).
>
> The condition file is the only other input required for Libra, so just
> specify it by name in the interface, and you should be good to go.
>
> Hope this helps.  Let us know if you have any other questions, and please
> report back on your TMT experience with Libra.  We would like to get a hold
> of the "standard" isotopic contribution factors so that we can include them
> as defaults in the GUI.
> --Luis
>
> On Thu, Jan 29, 2009 at 5:45 AM, Ruby <[email protected]> wrote:
>
> > Has anyone ever used Libra to analyze TandemMassTag (TMT) data
> > acquired on an Orbitrap?  I'm using the TPP via the SageN/Sorcerer
> > interface, and would love it if someone could tell me what the command
> > line would be appropriate for the "Xinteract options".
>
> > Thanks!
> > Rebekah
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