Hi guys, Back here after a long break :-) I want to use the TPP for quantitation of metabolic labeling experiments - both 14N/15N and 12C/ 13C. I have two questions:
1.) Is it possible to use 13C labeling with either XPress or ASAPRatio? I've found only options for 15N, so is there any chance to process 13C-labeled samples? 2.) How to perform the searches (Mascot in my case)? As far as I understood I have to search each sample twice - once for the light and once for the heavy peptides. That means to modify the masses and modification files on the Mascot server for every heavy search, so it is quite uncomfortable, especially if the Mascot server is shared with a lot of other people... With version 2.2, Mascot offers an option for metabolic labeling, resulting in the identification of light and heavy peptides in one run. Is it possible to process such a combined result file through the TPP? Thanks for any help! - Matthias --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [email protected] For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en -~----------~----~----~----~------~----~------~--~---
