On Apr 17, 2009, at 9:29 AM, spctools-discuss group wrote:

> Date: Thurs, Apr 16 2009 8:49 am
> From: brennmat
>
>
> Dear all
>
> I am an absolute newbie and I am not sure if I came to the right
> place... well, here's my question:
>
> I am looking for a tool that extracts chromatogram data from XCalibur
> *.raw files and converts them into a format that is readable by other
> software (e.g. Matlab or Octave). My *.raw files contain data from a
> GC/MS system which has both an MS and an ECD (electron capture
> detector; note that the data from the ECD does not contain massess,
> only signal intensity as a function of time).
>
> I can think of two ways to achieve this:
>
> 1. There is already a tool available that does what I need. This might
> be a converter that converts the XCalibur *.raw into a file that is
> readable by Matlab or Octave. Remember that I not only need the MS
> data, but also the ECD data which does not have any mass information.
> Otherwise I could use XCalibur/xconvert or ReAdW, which export to ANDI
> or mzXML. Is there a converter which also processes 'massless'
> chromatograms?
>
> 2. I might try to write some code in C/C++ that does what I need. This
> might be either a standalone program or a MEX or OCT binary that runs
> from within Matlab or Octave. In both cases I'd need some help to get
> me started on how to use the XCalibur DLL stuff in C/C++. I have never
> worked with C/C++ on Microsoft Windows and I've got no clue how to
> work with DLLs. Any pointers or code examples would be greatly
> appreciated.
>
> I'd be very grateful for any hints, comments and suggestions.
>
>
> Thanks
> Matthias

Dear all

This is an old thread, but I wanted to let the world know that I've found a
solution. Matt Chambers did a great job in extending the ProteoWizard tools
with support for the ECD sensor (THANKS Matt!!!!). I am now using msconvert
(one of the ProteoWizard tools) to convert the Xcalibur RAW file to an mzML
file. I then load the data from the mzML file into Octave (or Matlab). Just
in case my Octave/Matlab files might be useful to someone else I published
them on soureforge.net:
http://sourceforge.net/projects/matchromat
svn co https://matchromat.svn.sourceforge.net/svnroot/matchromat matchromat

Matthias

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