Hi,

I would like to continue an old thread:
http://groups.google.com/group/spctools-discuss/browse_thread/thread/4fd1ce8369511c8c/5b357a153c4a1b24?lnk=gst&q=viewing+spectrum#5b357a153c4a1b24,
which has already been taken off.

My problem is similar, but I have tried my best to verify that all the
places referring to the raw data "path" are consistent, as advised by
Jimmy in the thread. What I did was: I made a Tandem DB search at one
folder level (e.g. c:/Inetpub/wwwroot/ISB/data/OC/OGEmRPTD10/P00158),
generating a set of .raw.tandem files. But later, for the reason of
trying to combine 529 mzXML files together from separate folders, I
moved all the mzXML and associated .raw.tandem files to another folder
level (c:/Inetpub/wwwroot/ISB/data/OC/OGEmRPTD10/).

I know of this spectra viewing problem if the files were relocated, so
I used a text replacing application to ensure that all .raw.tandem
and .raw.tandem.params files with the string "c:/Inetpub/wwwroot/ISB/
data/OC/OGEmRPTD10/P00158" were replaced to "c:/Inetpub/wwwroot/ISB/
data/OC/OGEmRPTD10/". Only then I generated the .raw.tandem.pep.xml
files from the modified .raw.tandem files and then the
interact.pep.xml file.

But the error still persists: command "C:\Inetpub\tpp-bin\tar.exe -tzf
c:/Inetpub/wwwroot/ISB/data/OC/OGEmRPTD10/
HUO00159S00168GL05B01.raw.tandem.pep.tgz "*HUO00159S00168GL05B01.raw.
0659.0659.3.dta" > /dev/null" failed: Operation not permitted Error -
cannot read spectrum; tried direct .dta, from mzXML/mzData and
from .tgz

Below is an example of a spectra path that I tried to track:

Spectra URL   =

&Dta=c:/Inetpub/wwwroot/ISB/data/OC/OGEmRPTD10/
HUO00159S00168GL05B01.raw.tandem.pep/HUO00159S00168GL05B01.raw.
0660.0660.2.dta&

Interact.pep.xml file   =

<interact_summary filename="c:/Inetpub/wwwroot/ISB/data/OC/OGEmRPTD10/
OGEmRPTD10_Tandem_interact.pep.xml" directory="C:/Inetpub/wwwroot/ISB/
data/OC/OGEmRPTD10">
<inputfile name="HUO00159S00168GL05B01.raw.tandem.pep.xml"/>
<msms_run_summary base_name="c:/Inetpub/wwwroot/ISB/data/OC/OGEmRPTD10/
HUO00159S00168GL05B01.raw.tandem.pep"
<search_summary base_name="c:/Inetpub/wwwroot/ISB/data/OC/OGEmRPTD10/
HUO00159S00168GL05B01.raw.tandem.pep"
<parameter name="spectrum, path" value="c:/Inetpub/wwwroot/ISB/data/OC/
OGEmRPTD10/HUO00159S00168GL05B01.raw.mzXML"/>

HUO00159S00168GL05B01.raw.tandem.pep.xml file   =

 <parameter name="spectrum, path" value="c:/Inetpub/wwwroot/ISB/data/
OC/OGEmRPTD10/HUO00159S00168GL05B01.raw.mzXML"/>


Sorry for this long and unreadable email, but I really don't know what
else I might have missed out, and I would hate to redo the Tandem
search for 529 mzXML files. Would really appreciate some suggestions
and advice. Thanks in advance!


Bernard

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