Hi Chris,, AFAIK, X! Tandem isn't designed to run on profile mode data. If you are using the default "total peaks" setting then it will take only the 50 most intense samples (not peaks, because it assumes every data point is a peak). 50 samples are easily consumed by just a few peaks. You can increase the total peaks setting, but I expect that would kill the score discrimination because it's designed to look at peaks instead of samples. You should continue to acquire centroided MS/MS data or at least have some way to centroid it post-acquisition before sending it to X! Tandem.
-Matt chr12is wrote: > Greetings, > > I am encountering an error which I dont remember having troubles with > before. I used to acquire my data such that in MS/MS mode it would be > centroided, as in older versions of the TPP, filesizes over 4gb were > problematic. Since I have noticed this isnt a problem anymore in the > newer TPP versions, I am using 4.2.1, I have started acquiring in > continuum mode again for MS/MS. I have MassLynx 4.1 installed, and > have run centroided MS/MS files through the TPP on this computer with > no problems. > > However, if I take one of my new continuum files, it converts in > masswolf with no errors, I am not centroiding in masswolf, or using > gzip. When I take it to XTandem it gives me this error: > > X! TANDEM 2 (2007.07.01.3) > > Loading spectra ... > This application has requested the Runtime to terminate it in an > unusual way. > Please contact the application's support team for more information. > > > Any ideas whats happening? > > > --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [email protected] For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en -~----------~----~----~----~------~----~------~--~---
