It's best to ask all your questions here on the spctools-discuss list - you will reach more people who might know the answer, and any answers do you get will be there for others to read in the future. And of course the answer may already be in the spctools-discuss archives, so always start with a search.
Brian _____ From: [email protected] [mailto:[email protected]] On Behalf Of QuanZe He Sent: Wednesday, July 15, 2009 6:54 PM To: [email protected] Subject: [spctools-discuss] Re: Why not run Analyze Proteins when sample label by iTRAQ Dear Brian Pratt Yes, replace the tab with spaces is OK. Thanks a lot. Can I write the letter to you if I have some trouble in using TPP, if possibly. Best wish Bright 2009/7/16 Brian Pratt <[email protected]> It seems the crash is due to tab characters in the fasta file (probably legal, but ProteinProphet wasn't expecting it). Replace those tabs with spaces and it should all work. Brian _____ From: [email protected] [mailto:[email protected]] On Behalf Of QuanZe He Sent: Wednesday, July 15, 2009 1:14 AM To: [email protected] Subject: [spctools-discuss] Re: Why not run Analyze Proteins when sample label by iTRAQ Dear Brian Pratt I have zip up the pepXML and fasta files to Analyze_Proteins_Error.rar and send it to ftp://insilicos.serveftp.net/pub. Thank you very much. I will waiting your replay. Best wish Bright 2009/7/15 Brian Pratt <[email protected]> Hard to say what is happening there - you might zip up the pepXML and fasta files and FTP them to ftp://insilicos.serveftp.net/pub and I could take a quick look. Brian -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Bright Sent: Monday, July 13, 2009 10:52 PM To: spctools-discuss Subject: [spctools-discuss] Why not run Analyze Proteins when sample label by iTRAQ Hi everyone I use TPP v4.2 JETSTREAM rev 1, Build 200904071723 to analyse the sample which when label by iTRAQ but the Analyze Protein can't normal finish. The error report is following: running: "C:/Inetpub/tpp-bin/PepXMLViewer.cgi -I c:/Inetpub/wwwroot/ ISB/data/LiuXiaoHui/interact.pep.xml" command completed in 1 sec running: "C:/Inetpub/tpp-bin/ProteinProphet "interact.pep.xml" "interact.prot.xml" XML" ProteinProphet (C++) by Insilicos LLC and LabKey Software, after the original Perl by A. Keller (TPP v4.2 JETSTREAM rev 1, Build 200904071723 (MinGW)) (xml input) (report Protein Length) (using degen pep info) . . . reading in c:/Inetpub/wwwroot/ISB/data/LiuXiaoHui/ interact.pep.xml. . . . . . read in 1 1+, 83 2+, 20 3+, 0 4+, 0 5+, 0 6+, 0 7+ spectra with min prob 0.05 command "C:/Inetpub/tpp-bin/ProteinProphet "interact.pep.xml" "interact.prot.xml" XML" failed: Unknown error command "C:/Inetpub/tpp-bin/ProteinProphet "interact.pep.xml" "interact.prot.xml" XML" exited with non-zero exit code: -1073741676 QUIT - the job is incomplete command "C:\Inetpub\tpp-bin\xinteract.exe -Ninteract.pep.xml -p0.05 - l7 -Ofp -Lcondition.xml-1 c:/Inetpub/wwwroot/ISB/data/LiuXiaoHui/ F003217.pep.xml" failed: Unknown error Command FAILED Can you tell me the reasons why happend it. I will appreciate for your help. Thank your Bright --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [email protected] For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en -~----------~----~----~----~------~----~------~--~---
