Hello Robert,  It is a bit difficult from your message to get an
complete picture of your workflow.  In our workflow, when using
multiple search engines we combine the search result using the
iProphet tool.  Enabling the force fitting model is a very dangerous
approach.  Your final results could have many false positives unless
you include decoy sequences in your databas as an independent measure
of error in your final protein list.  -David

On 8/11/09, Robert <[email protected]> wrote:
>
> Concerning my previous post about "lost" peptides in  the TPP (X!
> Tandem/Peptide+ProteinProphet, v4.2)
>
> Checking "Force the fitting of the mixture model (bypass automatic
> mixture model checks)" indeed significantly increases the number of
> assigned peptides to a  protein and therefore the sequence coverage.
>
> Now also the peptides assigned to a protein by OMSSA are  included
>
> Regards, Robert
>
> >
>

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