We are proud to offer an update to the Trans-Proteomic Pipeline (TPP)
software, release 4.3.1 (4.3 revision 1).  The software is available for
Windows as well as linux (and unofficially) through osx source from all the
usual locations (please see the section below, "Getting the software").
 Most users are recommended to use the Windows installer, which installs and
configures the TPP and other required software (such as a webserver).  For
advanced users who need to customize the TPP, or for those who run
high-throughput experiments, you can download the source code.  Again, most
users don't need build from source, and should use the Windows installer.


Trans-Proteomic Pipeline (TPP) 4.3.1 release notes
 (Changes since the last official release, 4.3.0)

== Contents ==

- full mzML 1.1 and 1.0 support
- important note for using mscovert and PWIZ on Windows
- Petunia bug fix
- XPRESS bug fixes
- ProteinProphet bug fix
- mergeCharges utility memory usage improvement
- updatePaths utility bug fixes
- improved perl scripts
- known issues
- Getting the TPP software




== full mzML 1.1 and 1.0 support==

* mzML parsing code updated for full mzML 1.1 compatibility, including full
1.0 backwards compatiblity.  Includes a fix to X!Tandem's custom mzXML
parser, which now correctly handles mzML 1.0 and 1.1 data.




== important note for using mscovert and PWIZ on Windows==

* PWIZ and mscovert require the free "Microsoft Visual C++ 2008 SP1
Redistributable Package" to be installed on your computer before use.  You
can download this package from
http://www.microsoft.com/downloads/details.aspx?familyid=A5C84275-3B97-4AB7-A40D-3802B2AF5FC2&displaylang=en

* For processing Thermo RAW files, PWIZ and mscovert require the free
"MSFileReader" download, which can be found here:
http://sjsupport.thermofinnigan.com/public/detail.asp?id=586




== Petunia bug fix ==

* correct Analysis Pipeline now displays when selected (previously was
always displaying Sequest interface in 4.3.0)




== XPRESS bug fixes ==

* fixed bug which caused N15 metabolic labeling option to run incorrectly
(introduced when C13 option added).

* XPressCGIProteinDisplayParser.cgi bug fix:  a counter/index variable was
re-used in an inner loop causing outer loop to exit prematurely resulting in
the CGI not displaying peptide ratios from all input files; problem appears
to be present in all previous releases




== ProteinProphet bug fix ==

* handle missing description lines for IPI database searches




== mergeCharges utility memory usage improvement ==

* improved memory usage




== updatePaths utility bug fixes ==

* it will actually update root and database paths at the same time, if
specified.  Cleaned-up output too.




== improved perl scripts ==

* better handling of non-standard TPP installation location




== known issues ==

* mzWiff: charge state information is not recorded in output mzXML

* massWolf: charge state information is not recorded in output mzXML

* trapper: if dual-mode file is processed, the resulting file is possibly
always converted as centroid (even if centroid is not selected.)




== Getting the TPP software ==

* Download the TPP version 4.3.1 native windows installer
(TPP_Setup_v4_3_JETSTREAM_rev_1.exe) from the Sashimi SourceForge project
file release page:
  "http://sourceforge.net/projects/sashimi/files/";


* Everyone is encouraged to read and contribute to our wiki, at
  "http://tools.proteomecenter.org/wiki/";


* For guides to installing and using our software, please see our wiki:
  "http://tools.proteomecenter.org/wiki/index.php?title=Software:TPP";


* For downloading the source code, please go to the following link:
  "http://sourceforge.net/projects/sashimi/files/"; and find the 4.3.1 source
code .zip package;
or, check out the code directly from svn:
  "svn co
http://sashimi.svn.sourceforge.net/svnroot/sashimi/tags/release_4-3-1";

  For building from source, please refer to the readme file in TPP/src as
well as the wiki.




The TPP Team: Luis, David, Brian, and Natalie, plus all other developers who
contributed to this release from the ISB.  Thanks to developers and users
from the TPP's user community who provided feedback and code contributions.

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