Hi everyone
   I have a problem about using  ASAPRatio to analyse quantitative
data of 18O in TPP 4.31. In  "ASAPRatio Options" panel, I selected
"RUN ASAPRatio" and selected item "c" from "Changelabeled residues
to". At last clicked "Run XInteract" button to analyse
isotopiclabeling data by O18. The detail error message is showing in
following:

 run_in c:/Inetpub/wwwroot/ISB/data/HWJ; c:\Inetpub\tpp-bin\xinteract
-Ninteract.pep.xml -p0.05 -l7 -Of -A-lc-r0.5 c:/Inetpub/wwwroot/ISB/
data/HWJ/F002370.pep.xml

c:\Inetpub\tpp-bin\xinteract (TPP v4.3 JETSTREAM rev 0, Build
200908071234 (MinGW))

running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" "c:/
Inetpub/wwwroot/ISB/data/HWJ/F002370.pep.xml" -L"7""
 file 1: c:/Inetpub/wwwroot/ISB/data/HWJ/F002370.pep.xml
 processed altogether 14217 results


 results written to file c:/Inetpub/wwwroot/ISB/data/HWJ/
interact.pep.xml

 direct your browser to http://localhost/ISB/data/HWJ/interact.pep.shtml



command completed in 5 sec

running: "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
MINPROB=0.05 NOICAT"
 (MASCOT)
results for charge 1: 247 id tot and 49 adj scores
results for charge 1: 8.39417 adj_ion_mean and 13.7552 adj_ion_hom
mean 28.8496id mean0.907893 correlation (r)

...skipping 22 lines of output...
WARNING: Mixture model quality test failed for charge (5+).
WARNING: Mixture model quality test failed for charge (6+).
  model complete after 23 iterations
command completed in 7 sec

running: "C:/Inetpub/tpp-bin/ProphetModels.pl -i interact.pep.xml"
Analyzing interact.pep.xml ...
Parsing search results "c:/Inetpub/wwwroot/ISB/data/HWJ/F002370
(MASCOT)"...
  => Total of 7230 hits.
command completed in 2 sec

running: "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "interact.pep.xml"
-lc -r0.5"
cterm: 0 vs 0

command "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "interact.pep.xml" -
lc -r0.5" failed: Operation not permitted

command "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "interact.pep.xml" -
lc -r0.5" exited with non-zero exit code: 1
QUIT - the job is incomplete

command "c:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -
Of -A-lc-r0.5 c:/Inetpub/wwwroot/ISB/data/HWJ/F002370.pep.xml" failed:
Operation not permitted
Command FAILED


If you know how to deal with the problem please tell me

Thank

Bright
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