dear list,
i tried to process several files with spectrast but get this error
code which unfortunately tells me nothing :-(
hopefully one of you can help me out here ...
cheers,
andreas
p.s. the output of the xinteract run looked as follow:
/usr/local/apps/tpp/bin/xinteract (TPP v4.3 JETSTREAM rev 1, Build
200909181208 (linux))
running: "/usr/local/apps/tpp/bin/InteractParser
'/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.pep.xml'
'/IMSB/results/workflow/158/init/O08-10100_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10105_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10114_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10107_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10093_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10094_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10102_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10096_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10104_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10095_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10111_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10098_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10116_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10099_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10103_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10106_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10112_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10097_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10109_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10108_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10113_c.pep.xml'
'/IMSB/results/workflow/158/init/O08-10115_c.pep.xml' -L'7'"
file 1: /IMSB/results/workflow/158/init/O08-10100_c.pep.xml
file 2: /IMSB/results/workflow/158/init/O08-10105_c.pep.xml
file 3: /IMSB/results/workflow/158/init/O08-10114_c.pep.xml
file 4: /IMSB/results/workflow/158/init/O08-10107_c.pep.xml
file 5: /IMSB/results/workflow/158/init/O08-10093_c.pep.xml
file 6: /IMSB/results/workflow/158/init/O08-10094_c.pep.xml
file 7: /IMSB/results/workflow/158/init/O08-10102_c.pep.xml
file 8: /IMSB/results/workflow/158/init/O08-10096_c.pep.xml
file 9: /IMSB/results/workflow/158/init/O08-10104_c.pep.xml
file 10: /IMSB/results/workflow/158/init/O08-10095_c.pep.xml
file 11: /IMSB/results/workflow/158/init/O08-10111_c.pep.xml
file 12: /IMSB/results/workflow/158/init/O08-10098_c.pep.xml
file 13: /IMSB/results/workflow/158/init/O08-10116_c.pep.xml
file 14: /IMSB/results/workflow/158/init/O08-10099_c.pep.xml
file 15: /IMSB/results/workflow/158/init/O08-10103_c.pep.xml
file 16: /IMSB/results/workflow/158/init/O08-10106_c.pep.xml
file 17: /IMSB/results/workflow/158/init/O08-10112_c.pep.xml
file 18: /IMSB/results/workflow/158/init/O08-10097_c.pep.xml
file 19: /IMSB/results/workflow/158/init/O08-10109_c.pep.xml
file 20: /IMSB/results/workflow/158/init/O08-10108_c.pep.xml
file 21: /IMSB/results/workflow/158/init/O08-10113_c.pep.xml
file 22: /IMSB/results/workflow/158/init/O08-10115_c.pep.xml
processed altogether 99822 results
results written to file
/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.pep.shtml
command completed in 23 sec
running: "/usr/local/apps/tpp/bin/PeptideProphetParser
'/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.pep.xml'
DECOY=DECOY_ ACCMASS LEAVE PI INSTRWARN"
using Accurate Mass Bins
using pI
Using no error on different instrument types.
Using Decoy Label "DECOY_".
(SpectraST)
adding Accurate Mass mixture distr
adding pI mixture distr
init with SpectraST trypsin
MS Instrument info: Manufacturer: ThermoFinnigan, Model: LTQ Orbitrap
XL, Ionization: ESI, Analyzer: ITMS, Detector: EMT
PeptideProphet (TPP v4.3 JETSTREAM rev 1, Build 200909181208
(linux)) akel...@isb
read in 0 1+, 75687 2+, 21029 3+, 2960 4+, 146 5+, 0 6+, and 0 7+ spectra.
Initialising statistical models ...
Iterations: .........10.........20.........
model complete after 30 iterations
command completed in 79 sec
running: "/usr/local/apps/tpp/bin/ProphetModels.pl -i
/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.pep.xml"
Analyzing /IMSB/results/workflow/158/spectrast_xinteract/Xinteract.pep.xml ...
Reading Accurate Mass Model model +1 ...
Reading kernel density calc pI [pI] model +1 ...
Reading Accurate Mass Model model +2 ...
Reading kernel density calc pI [pI] model +2 ...
Reading Accurate Mass Model model +3 ...
Reading kernel density calc pI [pI] model +3 ...
Reading Accurate Mass Model model +4 ...
Reading kernel density calc pI [pI] model +4 ...
Reading Accurate Mass Model model +5 ...
Reading kernel density calc pI [pI] model +5 ...
Reading Accurate Mass Model model +6 ...
Reading kernel density calc pI [pI] model +6 ...
Reading Accurate Mass Model model +7 ...
Reading kernel density calc pI [pI] model +7 ...
Parsing search results "/home/andqua/O08-10100_c (SpectraST)"...
=> Total of 2389 hits.
Parsing search results "/home/andqua/O08-10105_c (SpectraST)"...
=> Total of 2321 hits.
Parsing search results "/home/andqua/O08-10114_c (SpectraST)"...
=> Total of 1933 hits.
Parsing search results "/home/andqua/O08-10107_c (SpectraST)"...
=> Total of 1949 hits.
Parsing search results "/home/andqua/O08-10093_c (SpectraST)"...
=> Total of 2660 hits.
Parsing search results "/home/andqua/O08-10094_c (SpectraST)"...
=> Total of 2756 hits.
Parsing search results "/home/andqua/O08-10102_c (SpectraST)"...
=> Total of 2743 hits.
Parsing search results "/home/andqua/O08-10096_c (SpectraST)"...
=> Total of 2813 hits.
Parsing search results "/home/andqua/O08-10104_c (SpectraST)"...
=> Total of 2457 hits.
Parsing search results "/home/andqua/O08-10095_c (SpectraST)"...
=> Total of 2889 hits.
Parsing search results "/home/andqua/O08-10111_c (SpectraST)"...
=> Total of 298 hits.
Parsing search results "/home/andqua/O08-10098_c (SpectraST)"...
=> Total of 454 hits.
Parsing search results "/home/andqua/O08-10116_c (SpectraST)"...
=> Total of 2116 hits.
Parsing search results "/home/andqua/O08-10099_c (SpectraST)"...
=> Total of 1861 hits.
Parsing search results "/home/andqua/O08-10103_c (SpectraST)"...
=> Total of 2690 hits.
Parsing search results "/home/andqua/O08-10106_c (SpectraST)"...
=> Total of 1997 hits.
Parsing search results "/home/andqua/O08-10112_c (SpectraST)"...
=> Total of 492 hits.
Parsing search results "/home/andqua/O08-10097_c (SpectraST)"...
=> Total of 2191 hits.
Parsing search results "/home/andqua/O08-10109_c (SpectraST)"...
=> Total of 743 hits.
Parsing search results "/home/andqua/O08-10108_c (SpectraST)"...
=> Total of 1959 hits.
Parsing search results "/home/andqua/O08-10113_c (SpectraST)"...
=> Total of 921 hits.
Parsing search results "/home/andqua/O08-10115_c (SpectraST)"...
=> Total of 2376 hits.
Warning: empty y range [0:0], adjusting to [0:1]
plot "/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.pep_IPPROB.tsv"
using 2:1 title "iProphet" with line lc 2 , x notitle with line lt 0
lc -1
^
"/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.pep_IPPROB.gp",
line 16: warning: Skipping data file with no valid points
command completed in 6 sec
running: "/usr/local/apps/tpp/bin/DatabaseParser
'/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.pep.xml'"
command completed in 1 sec
running: "/usr/local/apps/tpp/bin/RefreshParser
'/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.pep.xml'
'/IMSB/data/databases/swissprot_57.7/sp_9606.fasta'"
- Building Commentz-Walter keyword tree... - Searching the tree...
- Linking duplicate entries... - Printing results...
command completed in 14 sec
running: "/usr/local/apps/tpp/cgi-bin/PepXMLViewer.cgi -I
/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.pep.xml"
command completed in 6 sec
running: "/usr/local/apps/tpp/bin/ProteinProphet
'/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.pep.xml'
'/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.prot.xml'
XML"
ProteinProphet (C++) by Insilicos LLC and LabKey Software, after the
original Perl by A. Keller (TPP v4.3 JETSTREAM rev 1, Build
200909181208 (linux))
(xml input) (report Protein Length) (using degen pep info)
. . . reading in
/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.pep.xml. . .
. . . read in 0 1+, 33817 2+, 8212 3+, 926 4+, 53 5+, 0 6+, 0 7+
spectra with min prob 0.05
INFO: mu=4.69978e-06, db_size=90930969
protein probabilities written to file
/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.prot.xml
direct your browser to
http://cal//IMSB/results/workflow/158/spectrast_xinteract/Xinteract.prot.shtml
command completed in 71 sec
running: "/usr/local/apps/tpp/bin/ProtProphModels.pl -i
/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.prot.xml"
Illegal division by zero at /usr/local/apps/tpp/bin/ProtProphModels.pl line 231.
Analyzing /IMSB/results/workflow/158/spectrast_xinteract/Xinteract.prot.xml ...
command "/usr/local/apps/tpp/bin/ProtProphModels.pl -i
/IMSB/results/workflow/158/spectrast_xinteract/Xinteract.prot.xml"
exited with non-zero exit code: 6400
QUIT - the job is incomplete
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