Sounds like you may also need to set the path for whatever user is running
the apache process.

Brian

(or maybe you already figured that out - this got to my inbox just today but
you posted 4 days ago?)

On Tue, Nov 17, 2009 at 12:47 PM, Austin <[email protected]> wrote:

> Hi Everyone,
>
> I started setting up the TPP on Ubuntu 9.04 / Apache2 over the weekend
> following the instructions found at
>
> http://tools.proteomecenter.org/wiki/index.php?title=TPP_4.2.1:_Installing_on_Ubuntu_9.04
> ,
> and have had a few minor issues (so far). The setup seems to have gone
> fine, and I can log into /tpp/cgi-bin/tpp_gui.pl and all that jazz.
>
> The first issue I encountered in creating PepXML files from .out
> files. When I attempt to do the processing through the GUI, all of the
> files fail with the error:
>
>    sh: Out2XML: not found
>    END OUTPUT
>    RETURN CODE:32512
>
> and no .pep.xml files are produced. However, if I run the same command
> in a terminal:
>
> $> cd /usr/local/apps/ISB/data/Tutorial; Out2XML /usr/local/apps/ISB/
> data/Tutorial/raft4041 1 -P/usr/local/apps/ISB/data/Tutorial/
> sequest.params -Etrypsin
>
> there are no issues. I've added /usr/local/apps/tpp/bin to my $PATH
> variable, and this seems to work for everything except the TPP GUI.
> Any ideas?
>
> The second issue I've encountered is when trying to view the .pep.xml
> files through the PepXML viewer. At the top of the page I get the
> error:
>
>    Messages
>  Unable to access xml file via webserver, so cannot check xml file
> for changes ()
>  Check webserver configuration if desired.
>  (URL: undefined)
>
> The peptides get loaded, so it seems that the xml parsing goes as
> planned. However, the page is unable to load the stylesheet and the
> "PeptideAtlas" images. If I try to get to that image directly (http://
> localhost/tpp/html/images/pa_tiny.png) I receive a 500 internal server
> error. Any ideas? I've sifted through the virtual host configuration a
> bit and haven't found any obviously offending lines. Any help at all
> will be greatly appreciated!
>
>  - Austin
>
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>
>

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