Dear All,

I am trying to analyze InSpecT results on pepxml format with TPP. I
copied below the error message that TPP generates. Any help will be
greatly appreciated.

Cheers,
Cristian



 run_in c:/Inetpub/wwwroot/ISB/data/try; c:\Inetpub\tpp-bin\xinteract
-Ninteract0209.pep.xml -p0.05 -l7 -O c:/Inetpub/wwwroot/ISB/data/try/
writeto.pep.xml
--------------------------------------------------------------------------------


c:\Inetpub\tpp-bin\xinteract (TPP v4.3 JETSTREAM rev 1, Build
200909091257 (MinGW))

running: "C:/Inetpub/tpp-bin/InteractParser "interact0209.pep.xml" "c:/
Inetpub/wwwroot/ISB/data/try/writeto.pep.xml" -L"7""
 file 1: c:/Inetpub/wwwroot/ISB/data/try/writeto.pep.xml
 processed altogether 10897 results


 results written to file c:/Inetpub/wwwroot/ISB/data/try/
interact0209.pep.xml

 direct your browser to http://localhost/ISB/data/try/interact0209.pep.shtml



command completed in 8 sec

running: "C:/Inetpub/tpp-bin/PeptideProphetParser
"interact0209.pep.xml" MINPROB=0.05"
 (InsPecT)
WARNING!! The discriminant function for Inspect is not yet complete.
It is presented here to help facilitate trial and discussion.
Reliance on this code for publishable scientific results is not
recommended.
WARNING: Inspect only support semi-parametric PeptideProphet
modelling, which relies on a DECOY search.
WARNING: Inspect only support semi-parametric PeptideProphet
modelling, which relies on a DECOY search.
WARNING: Inspect only support semi-parametric PeptideProphet
modelling, which relies on a DECOY search.
WARNING: Inspect only support semi-parametric PeptideProphet
modelling, which relies on a DECOY search.
WARNING: Inspect only support semi-parametric PeptideProphet
modelling, which relies on a DECOY search.
WARNING: Inspect only support semi-parametric PeptideProphet
modelling, which relies on a DECOY search.
WARNING: Inspect only support semi-parametric PeptideProphet
modelling, which relies on a DECOY search.
init with InsPecT None
MS Instrument info: Manufacturer: UNKNOWN, Model: ESI-ION-TRAP,
Ionization: UNKNOWN, Analyzer: UNKNOWN, Detector: UNKNOWN

 PeptideProphet  (TPP v4.3 JETSTREAM rev 1, Build 200909091257
(MinGW)) akel...@isb
 read in 938 1+, 4815 2+, 3088 3+, 0 4+, 0 5+, 0 6+, and 0 7+ spectra.
Initialising statistical models ...
Iterations: .........10.........20

command "C:/Inetpub/tpp-bin/PeptideProphetParser
"interact0209.pep.xml" MINPROB=0.05" failed: Unknown error

command "C:/Inetpub/tpp-bin/PeptideProphetParser
"interact0209.pep.xml" MINPROB=0.05" exited with non-zero exit code:
-1073741819
QUIT - the job is incomplete

command "c:\Inetpub\tpp-bin\xinteract -Ninteract0209.pep.xml -p0.05 -
l7 -O c:/Inetpub/wwwroot/ISB/data/try/writeto.pep.xml" failed: Unknown
error
Command FAILED

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