Hi,

I've been using both trapper and msconvert to convert Agilent
MassHunter ".d" data to mzXML. I'm using msconvert from the
proteowizard 1.6.0 distribution, and I'm using the standalone trapper
4.3.1 from sashimi.

I've noticed that the msconvert output is structured differently from
the trapper output, and appears to break at least two rules specified
in the mzXML 2.1 tutorial (http://sashimi.sourceforge.net/
schema_revision/mzXML_2.1/Doc/mzXML_2.1_tutorial.pdf).

(1) Scan numbers (as per the scan/@num attribute) are supposed to
start from 1, and ascend sequentially (presumably without skipping).
The msconvert output numbering appears to be arbitrary (for me, it
starts at 2273 and ascends with gaps).

(2) Scan elements representing MS2 scans are supposed to be nested
within the elements representing their precursor MS1 scans. The
msconvert output does not perform this nesting.

The trapper output appears compliant.

Are these differences causes for concern? Is it possible that SPC
tools (or others) might function incorrectly when processing msconvert-
generated mzXML files?

Thanks,
bio.x2y

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