Hi All,

I'd like to have some opinions on how to validate a dataset with
no_enzyme specificty using PeptideProphet ?

Until now it looks like it's difficult to get a model working. I
converted to pepxml using -Enonspecific from Mascot or Sequest by it
looks like the model has difficulty. I'm wondering who has experience
with nonspecific samples and validation. Should I turn off some
standard features like -OFNM ???

Thanks for any help.

Alex

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