so my question is, what do the 'peptideprophet probability' and
'interprophet probability' in pep.xml, and 'initial probability' and
'nsp_adjusted probability' in prot.xml stand for?
Thanks a lot!

Amy

On Apr 19, 6:08 pm, grassworm <[email protected]> wrote:
> hello everyone,
>
> i'm starting to use TPP and would like to thanks the authors for this
> great program! i yet have some questions about the meanings of several
> probabilities in the pep.xml and prot.xml files.
>
> first there's this 'peptideprophet_result probability' in the in the
> pep.xml file, in lines that look like this:
> <peptideprophet_result probability="0.6908"
> all_ntt_prob="(0.0000,0.6967,0.6908)">.
> at first i thought it was the same as the 'initial probability' in the
> corresponding prot.xml (e.g.
> <peptide peptide_sequence="HGVQELEIELQSQLSK"
> initial_probability="0.9990" nsp_adjusted_probability="0.9999"
> weight="1.00" is_nondegenerate_evidence="Y" n_enzymatic_termini="2"
> n_sibling_peptides="8.20" n_sibling_peptides_bin="6" n_instances="23"
> exp_tot_instances="22.05" is_contributing_evidence="Y">), and that the
> interprophet_result probability in lines like
> <interprophet_result probability="0.518555"
> all_ntt_prob="(0,0.525484,0.518555)">
> are the same as the nsp_adjusted_probability in the prot.xml. however
> i checked the values of several peptides and found that they don't
> always match. the peptide values are often smaller than the ones in
> the prot.xml.
>
> i'm not sure if this has been discussed before. appologies if i've
> brought up a toooold question, and please direct me to the relevent
> discussions or documentations. thanks for your help!
>
> Amy
>
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