Dear all, I got a problem when I did PeptideProphet analysis on X!Tandem results as following messages:
------------------------------------------------------------------------------------------------------------------------------------------------- run_in c:/Inetpub/wwwroot/ISB/data; c:\Inetpub\tpp-bin\xinteract - Ninteract.pep.xml -p0.05 -l7 -O c:/Inetpub/wwwroot/ISB/data/ D0420-37F.tandem.pep.xml c:\Inetpub\tpp-bin\xinteract (TPP v4.3 JETSTREAM rev 1, Build 200909091257 (MinGW)) running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" "c:/ Inetpub/wwwroot/ISB/data/D0420-37F.tandem.pep.xml" -L"7"" file 1: c:/Inetpub/wwwroot/ISB/data/D0420-37F.tandem.pep.xml processed altogether 7153 results results written to file c:/Inetpub/wwwroot/ISB/data/interact.pep.xml direct your browser to http://localhost/ISB/data/interact.pep.shtml command completed in 2 sec running: "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml" MINPROB=0.05" (X! Tandem (k-score)) init with X! Tandem (k-score) trypsin MS Instrument info: Manufacturer: Thermo Scientific, Model: LTQ Orbitrap, Ionization: NSI, Analyzer: FTMS, Detector: unknown ...skipping 18 lines of output... => Total of 0 hits. command completed in 0 sec running: "C:/Inetpub/tpp-bin/DatabaseParser "interact.pep.xml"" command completed in 0 sec running: "C:/Inetpub/tpp-bin/RefreshParser "interact.pep.xml" "c:/ Inetpub/wwwroot/ISB/data/dbase/22.H_salinarium.fasta"" - Searching the tree... - Linking duplicate entries... - Printing results... - Building Commentz-Walter keyword tree...command completed in 1 sec running: "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I c:/Inetpub/ wwwroot/ISB/data/interact.pep.xml" command "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I c:/Inetpub/ wwwroot/ISB/data/interact.pep.xml" failed: Unknown error command "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I c:/Inetpub/ wwwroot/ISB/data/interact.pep.xml" exited with non-zero exit code: 255 QUIT - the job is incomplete command "c:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -O c:/Inetpub/wwwroot/ISB/data/D0420-37F.tandem.pep.xml" failed: Unknown error Command FAILED -------------------------------------------------------------------------------------------------------------------------------------------- I''m sure that there are peptides with probabilities > 0.05 in interact.pep.xml. But I always cannot run PeptideProphet successfully. What happened to that? Is there something I missed? Thanks in advance. H.W. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
