Dear all, I am a freshman in bio-informatics and just start to use TPP. I have just installed TPP v4.4 together with its GUI interface (Petunia) on my Windows 7 laptop and went through the tutorial on the SPC Tools Wiki.
Interestingly, when following the tutorial, many things did not work properly in Petunia. For example, after having generated the pepXML and tried to generate Pep3D image in the PepXML Viewer, the Pep3D Image for LC-ESI-MS Data did not display - a broken image link was presented on the pane. The similar situation happened when I tried to use COMET Spectrum View and CID Spectrum Display as well - everything was listed properly; however, no image was displayed. Additionally, when clicking a protein index in the "protein" column in the PepXML viewer, the COMET Sequence Viewer did not display any useful information such as protein serial no, observed peptides, and in-silico digestion, instead an error message "Error - cannot open/ dbase/IPI/ ipi.HUMAN.fasta.v2.31 (No such file or directory)" was presented. As I have strictly followed the SPC's tutorial, I was wondering whether I had done something wrong or everyone else encountered the same issue as I did... If so, PLEASE, can you help me out and enlighten me how to resolve these issues! Many thanks in advance, Gash -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
