I have trouble to convert Masslynx's MSe data file to mzXML then to .mgf file with TPP. Here is the command I used: masswolf --mzXML --nolockspray --MSe C:\data.raw C:\data.mzXML
The output file data.mzXML can be viewed with InsilicoView and it looked ok. However, when TPP was used to convert .data.mzXML to .mgf, there was no peak in it. In MSe mode, low energy was set at 2 and high energy was set to 20-30V. I don't have much experience on Masslynx software. Is it possible that some settings in MSe mode was not correct? or is it possible masswolf requires some special settings? any comment is appreciated. By the way, I have no problem to convert masslynx's DDA data file to mzXML then to .mgf using TPP. Mascort search with this .mgf file yielded positive results. In processing DDA data, masswolf command was masswolf --mzXML --nolockspray C:\data.raw C:\data.mzXML -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
