Hello, Is it possible to construct a metabolically-labelled library using SpectraST, first starting with a 12C all-light library? We've tried making a user-mod file with the appropriate mass shifts (http://pastebin.com/P5fQ43ZV).
The library starts out with 3226 entries. When a modification is given with the -cx command, SpectraST exclusively returns entries that contain that modification. For heavy Ala, this reduces the library size to 2903. When multiple modifications are included with the -cx'' command, the problem is exacerbated as the list of sequences is narrowed even more. For both heavy Ala and Asp, the library shrinks to 2379. Here's an attempt to install one, then two modifications. You can see that the library size decreases. http://pastebin.com/1RLqRwAR Is there a way to request all 20 modifications without withholding peptides that don't have all 20 amino acids? Thank you! Vadim -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at http://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
