I'm using the Amazon Cloud to run the TPP. I ran an X!tandem search yesterday, which appeared to process fine, and subsequently converted all *.tandem *files to *.pep.xml* files for use in PeptideProphet, which also appeared to work fine. Initially the xinteract process (all defaults run through, using Petunia for the TPP) appeared to work fine, with my first four files appearing to go through, but my 5th file appears to cause a segmentation fault. Has anyone had a similar problem? This is a rerun of an analysis we did back in December with TPP 4.6.2. Everything but our database is identical to our former search, although it has recently been annotated. Here are the logs:
*EXECUTING: cd /mnt/tppdata/MO3_1; xinteract -NMO3_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_Runs1thru3_interact.pep.xml -p0.05 -l7 -PPM -O M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_01.2014_06_04_23_00_51.t.tandem.pep.xml M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_02.2014_06_05_00_08_27.t.tandem.pep.xml M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_03.2014_06_05_00_46_54.t.tandem.pep.xml M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082013_Run2_02.2014_06_05_01_39_09.t.tandem.pep.xml M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082013_Run2_03.2014_06_05_02_32_02.t.tandem.pep.xml M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082013_Run2_04.2014_06_05_03_03_01.t.tandem.pep.xml MO3_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082513_Run3_02.2014_06_05_03_42_49.t.tandem.pep.xml MO3_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082513_Run3_03.2014_06_05_04_36_54.t.tandem.pep.xml MO3_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082513_Run3_04.2014_06_05_05_02_56.t.tandem.pep.xml * xinteract (TPP v4.7 POLAR VORTEX (AMI) rev 1, Build 201405271720 (linux)) PPM mode in Accurate Mass Model ... running: "/opt/tpp/bin/InteractParser '/opt/tpp/tmp/VBLNyi/MO3_1/MO3_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_Runs1thru3_interact.pep.xml' 'M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_01.2014_06_04_23_00_51.t.tandem.pep.xml' 'M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_02.2014_06_05_00_08_27.t.tandem.pep.xml' 'M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_03.2014_06_05_00_46_54.t.tandem.pep.xml' 'M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082013_Run2_02.2014_06_05_01_39_09.t.tandem.pep.xml' 'M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082013_Run2_03.2014_06_05_02_32_02.t.tandem.pep.xml' 'M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082013_Run2_04.2014_06_05_03_03_01.t.tandem.pep.xml' 'MO3_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082513_Run3_02.2014_06_05_03_42_49.t.tandem.pep.xml' 'MO3_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082513_Run3_03.2014_06_05_04_36_54.t.tandem.pep.xml' 'MO3_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082513_Run3_04.2014_06_05_05_02_56.t.tandem.pep.xml' -L'7'" file 1: M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_01.2014_06_04_23_00_51.t.tandem.pep.xml SUCCESS: CORRECTED data file /mnt/tppdata/MO3_1/M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_01.mzXML.gz in msms_run_summary tag ... file 2: M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_02.2014_06_05_00_08_27.t.tandem.pep.xml SUCCESS: CORRECTED data file /mnt/tppdata/MO3_1/M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_02.mzXML.gz in msms_run_summary tag ... file 3: M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_03.2014_06_05_00_46_54.t.tandem.pep.xml SUCCESS: CORRECTED data file /mnt/tppdata/MO3_1/M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_03.mzXML.gz in msms_run_summary tag ... file 4: M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082013_Run2_02.2014_06_05_01_39_09.t.tandem.pep.xml SUCCESS: CORRECTED data file /mnt/tppdata/MO3_1/M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082013_Run2_02.mzXML.gz in msms_run_summary tag ... file 5: M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082013_Run2_03.2014_06_05_02_32_02.t.tandem.pep.xml Segmentation fault (core dumped) command "/opt/tpp/bin/InteractParser '/opt/tpp/tmp/VBLNyi/MO3_1/MO3_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_Runs1thru3_interact.pep.xml' 'M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_01.2014_06_04_23_00_51.t.tandem.pep.xml' 'M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_02.2014_06_05_00_08_27.t.tandem.pep.xml' 'M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_050213_Run1_03.2014_06_05_00_46_54.t.tandem.pep.xml' 'M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082013_Run2_02.2014_06_05_01_39_09.t.tandem.pep.xml' 'M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082013_Run2_03.2014_06_05_02_32_02.t.tandem.pep.xml' 'M03_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082013_Run2_04.2014_06_05_03_03_01.t.tandem.pep.xml' 'MO3_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082513_Run3_02.2014_06_05_03_42_49.t.tandem.pep.xml' 'MO3_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082513_Run3_03.2014_06_05_04_36_54.t.tandem.pep.xml' 'MO3_1_Normal_Lyses_Buff_SCX_250uL_6hr_QExactive_082513_Run3_04.2014_06_05_05_02_56.t.tandem.pep.xml' -L'7'" exited with non-zero exit code: 35584 QUIT - the job is incomplete *Command FAILED* RETURN CODE:35584 -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at http://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
