Dear Luis,

That was extremely clear. Thanks a lot for the clarification!!!

Best
Kostas


On Thursday, August 28, 2014 11:52:53 AM UTC+3, Konstantinos Vougas wrote:
>
> Dear All,
>
> I have created a condition.xml file for the iTRAQ-8plex according to 
> Thermo's instructions (I am using an Orbitrap Elite) for the correction 
> values (see screenshot).
>
> I am also attaching the created condition.xml file.
>
> What I do not get and I need your help is the logic behind the "affected 
> mz" number.
> For instance
>
> 1.
> <contributingMz value="1">
>       <affected mz="2" correction="0.018" />
>       <affected mz="3" correction="0" />
>     </contributingMz>
>
> This I suppose refers to 113. According to the screenshot I sent you the 
> 3rd column (% of +1) and not the second (% of -1) has the value of 1.8%. 
> Why is  
>
> <affected mz="2" correction="0.018" />
>       <affected mz="3" correction="0" />
> ??
>
>
> 2. 
> <contributingMz value="7">
>       <affected mz="5" correction="0" />
>       <affected mz="6" correction="0.0259" />
>       <affected mz="8" correction="0" />
>     </contributingMz>
>
> This I suppose refers to 119. Why is the correction of 2.59% applied to 
> affected mz="6" and what does 6 represent? Shouldn't it be 1-4 as the 
> columns?
>
> and finally 
>
> 3. 
> <contributingMz value="8">
>       <affected mz="7" correction="0" />
>
> This I suppose refers to 121. My correction of 3.58% for "% of -1" which 
> is the 2nd column is nowhere to be found. Instead I get an affected mz="7" 
> correction="0"!!!
>
>
> Can anybody explain this?
>
>
>
> Thanks in advance
> K. Vougas
>
>

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