Hi I would like to ask you for help. I am using TPP for reanalysis of my 4plex iTRAQ data (LC-MS(MALDI TOF/TOF). Proteins identification goes fine, but when I am trying to use LIbra to obtain quantitative data I always get no result. There is an error. All parameters look fine, generated conditions file is correct, my iTRAQ labeling is complete (checked), all files localizations are ok. I start search with mzxml files (including itraq modification in the protocol for x tandem, then pep.xml file is ok. While running Libra I get such error.
running: "C:/Inetpub/tpp-bin/LibraPeptideParser "interact.pep.xml" -ccondition.xml" command completed in 1 sec running: "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I c:/Inetpub/wwwroot/ISB/data/parameters/interact.pep.xml" command "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I c:/Inetpub/wwwroot/ISB/data/parameters/interact.pep.xml" failed: Unknown error command "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I c:/Inetpub/wwwroot/ISB/data/parameters/interact.pep.xml" exited with non-zero exit code: -1073741819 QUIT - the job is incomplete Is it possible that MALDI data is not compatible with this software? I will be grateful for any help. Thanks Aleks -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at http://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
