Hi, I'm using TPP (4.8.0) to process Mascot searches (2.5.1). I can successfully convert the *.DAT file to a pepXML, but when I open the pepXML I cannot then select the ion2 to view the MS/MS spectra. I get the following error;
command "c:\Inetpub\wwwroot\..\tpp-bin\tar.exe -tzf c:/Inetpub/wwwroot/ISB/data/IS_all_01.tgz "*Fri_Aug_14_15-47-05_2015.spectrum1001.00000.00000.2.dta" > /dev/null" failed: Operation not permitted I used an MGF for searching, but I made that from an mzXML file. The process was WIFF -> mzML (AB data converter) -> mzXML (msconvert) -> MGF (msconvert). I used the mzXML as I need it for later analysis. The mzXML is not in the same directory as the DAT file during the DAT -> pepXML conversion process, nor is it there when I click the ions2 link. Is this relevant? For the above example, I can open the tgz file, get the right scan number, open it and see mass and intensity data. So it's there. The tgz mentioned above is also in the location stated. So why isn't this working? All suggestions gratefully received... When convert the DAT to a pepXML I do a get a few error messages about spectra not being found eg; Warning: could not find scan numbers of spectrum IS_all_01.7890.7890.2File:,NativeID:scan=7890 Set to 0000Warning: could not find scan numbers of spectrum IS_all_01.6844.6844.2File:,NativeID:scan=6844 Set to 0000Warning: could not find scan numbers of spectrum IS_all_01.6857.6857.2File:,NativeID:scan=6857 Set to 0000Warning: could not find scan numbers of spectrum IS_all_01.6258.6258.2File:,NativeID:scan=6258 Set to 0000Warning: could not find scan numbers of spectrum IS_all_01.6605.6605.2File:,NativeID:scan=6605 Set to 0000Warning: could not find scan numbers of spectrum IS_all_01.6509.6509.2File:,NativeID:scan=6509 Set to 0000 I'm not so worried about a few failing spectra, it's more that I can't see the spectra of the vast majority of my data. Thanks, Peter -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at http://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
