Hello Ryan

It may make it easier to troubleshoot the issues if you post the actual
output generated by the program.

Thank you,
David
On Dec 9, 2015 10:16 AM, "Ryan Lumpkin" <[email protected]> wrote:

> Hello all,
>
> Thank you in advance for your help. I have been using MS-GF+ to search
> spectra prior to analysis with TPP. I had to create a new set of scoring
> parameters for MS-GF+ in order to handle Orbitrap Fusion combined CID/ETD
> data, however after doing so, Peptide Prophet always errors out with 0 hits
> following the accurate mass model and PepXMLViewer crashes. I am aware that
> this can be indicative of bad data, yet my previous scoring parameters had
> no problem dealing with this same dataset. I'm under the impression that
> this is an issue with the settings used to generate those new scoring
> parameters with MS-GF+, but I unfortunately I have not found a better
> location to seek assistance with this.
>
> If anyone could point me in the right direction for resolving this, I
> would greatly appreciate it.
>
> Ryan Lumpkin
> Graduate Student
> University of California, San Diego
>
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