Hi Shiliang, where are your mzML files on your local machine?


I think that Petunia [Files] [Update Paths] is the right thing to do, but
you need to have the mzML files in that same directory to work I think.



So, in the same directory as you have:

LD-CS-150324_Medimmune_P12_Expt8_F10_151206172438.pep.xml

  (or perhaps the interact.pep.xml file)

Also have:

LD-CS-150324_Medimmune_P12_Expt8_F10_151206172438.mzML



And then run Petunia [Files] [Update Paths]. Did you try that?



Or where are you mzML files located?



If that’s what you had, maybe someone who understands [Update Paths] better
can help.



Regards,

Eric









*From:* [email protected] [mailto:
[email protected]] *On Behalf Of *Shiliang Wang
*Sent:* Thursday, February 25, 2016 6:41 PM
*To:* spctools-discuss <[email protected]>
*Subject:* [spctools-discuss] problem to open .pepXML file generated by TPP
twa with xml viewer



HI,



          I ran database search on TPP twa, and got the .pepXML file, I was
able to open the .pepXML file and also able to open the spectrum data by
clicking the IONS2 of a peptide, Every thing worked well. After I
downloaded the .pepXML and other files to a local directory, I was able to
open .pepXML file. However, when I tried to open the spectrum data by
clicking the IONS2 of a peptide, I got the following  error message.



          I was suggested to update paths in .pepXML using Petunia [Files]
[Update Paths]. The only option is to update database path. I updated it.
When I tried to open spectrum data in .pepXML table, I got the same error
message.



          Anyone can give me detail instruction? Which file need to be
updated for paths? the .pepXML file or the parameter file? Do I need to put
the .pepXML file in a particular folder or not?



          Thank you very much.



          Shiliang



command "c:\Inetpub\wwwroot\..\tpp-bin\tar.exe -tzf
/mnt/tppdata/SLE/LD-CS-150324_Medimmune_P12_Expt8_F10_151206172438.tgz
"*LD-CS-150324_Medimmune_P12_Expt8_F10_151206172438.00469.00469.2.dta" >
/dev/null" failed: Operation not permitted





   Messages

·  Error - cannot read spectrum; tried direct .dta, from mzML/mzXML/mzData
and from .tgz

·  Verify that file path is correct.

·  *LD-CS-150324_Medimmune_P12_Expt8_F10_151206172438.00469.00469.2.dta

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