Hi All, The MCP publication is now in the May issue: Plubell, D.L., Wilmarth, P.A., Zhao, Y., Fenton, A.M., Minnier, J., Reddy, A.P., Klimek, J., Yang, X., David, L.L. and Pamir, N., 2017. Extended multiplexing of TMT labeling reveals age and high fat diet specific proteome changes in mouse epididymal adipose tissue. Molecular & Cellular Proteomics, 16:873-890.
Cheers, Phil On Thursday, April 6, 2017 at 9:25:10 AM UTC-7, Phil wrote: > > Hi All, > I would like to bring attention to a new paper (I am a co-author) on > extended TMT multiplexing: > > > http://www.mcponline.org/content/early/2017/03/21/mcp.M116.065524.full.pdf+html > > <http://www.google.com/url?q=http%3A%2F%2Fwww.mcponline.org%2Fcontent%2Fearly%2F2017%2F03%2F21%2Fmcp.M116.065524.full.pdf%2Bhtml&sa=D&sntz=1&usg=AFQjCNHlqiTZrA5ogcTtpB-wLIjIOLaFLA> > > New instrument methods and 10-plex capacity are amazing improvements. > However, analysis examples are hard to come by. The methodology does not > yet use TPP but could probably be adapted to do so. The important parts of > the work are the experimental design itself. We do two channels for the > pooled standards because an average of two is a lot better than a single > measurement. It only drops the number of biological samples per TMT by 11% > (8 instead of 9). The IRS (happy tax time!) method is critical to avoiding > ratios. Ratios are more difficult to use in downstream statistical tests. > Combining PSMs into total protein reporter ion intensities greatly > simplifies analysis. Of course, that may not always be an option in many > experiments (phospho peptides come to mind). If anyone has questions, > please email me (wilmarth "at" ohsu.edu). > Cheers, > Phil W. > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
