Hi Everyone, I am analyzing peptides from cells that have been treated with a UV crosslinker attached to a small molecule (ABPP method). This results in mono-linked peptides. Because the UV cross-linker is not residue specific, I am forced to search the data in comet with a modification of 639.3118 Da on any amino acid other than those with fixed modifications. I am able to process the output files until the iProphet step, but am running into command failure at PTM Prophet. Is there a maximum limit on the number of amino acids used at once in a single line entry for a modification? I have attached screenshots of the parameters used and the command failure output.
Thanks! -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
