I posted this before but looks like the post did not go through so resubmitting:
1. When creating an unidentified spectral library, the read-out always shows the number of "non-peptide" spectra and "peptide" spectra are 0. This was confirmed using a defined peptide sample. Is this a read-out a simple format error or other? The command line is below: >spectrast -cNC:\TPP\data\Spectral\libraryname_unid C:\TPP\data\Spectral\Acqfilename.mzML 2. When using the subtract function (cJS = Subtraction. Only include peptide ions in the first file that are not present in any of the other files.), what is the ppm error threshold to assign ions between the files to be the same for subtraction? When use this function I see no difference in the number of spectra before or after subtraction. This is confirmed using a defined peptide sample. The command line is below: >>spectrast -cNC:\TPP\data\Spectral\Subtractionlibrary_unid -cJSC:\TPP\data\Spectral\Sample_unid.splib C:\TPP\data\Spectral\Blank_unid.splib Many thanks!! -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
