Please can someone advise - is it recommended to include decoys in my 
database when using X! for searches then peptide/protein prophet in TPP?


As I understand, X! recommends not including decoys in searches (when used 
standalone) – however when I analyse X! identified peptides using 
peptide/protein prophet, I assume that I will need decoy matches to help 
pin down the correct distribution?


Thanks

Pete

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