Hi David,

Great - I'll give that a go.

Thanks,
Alastair

Am Dienstag, 13. November 2018 17:47:41 UTC+1 schrieb David Shteynberg:
>
> Hello Alastair,
>
> The parameter you want for PeptideProphetParser is called MASSWIDTH=  .  I 
> have gotten good results setting it to 1000 for +-500Da MSFragger search 
> analysis.  
>
> Cheers,
> David 
>
> On Tue, Nov 13, 2018, 4:44 AM alastair.skeffington via spctools-discuss <
> spctools...@googlegroups.com <javascript:> wrote:
>
>> Hello,
>>
>> In the MSFragger publication the authors adopt an extended mass model 
>> when processing the data with peptide prophet which allows 
>> mass-shift-dependent FDR estimation. 
>>
>> I don't really see how you could implement this with the standard 
>> versions of peptide prophet - is the version of peptide prophet they used 
>> available? 
>>
>> In addition, is this a valid option for a small dataset resulting in a 
>> couple of thousand PSMs only? The number of PSMs per dM bin might be quite 
>> small...
>>
>> Any help would be much appreciated,
>>
>> Alastair
>>
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