Hi Eric, The port 10401 works fine. However, there is no Petunia showing up. When calling http://localhost:10401/ from the Firefox Browser the "Apache2 Ubuntu Default Page" appears. The host system is Fedora 29. To me it seems like a problem in the Apache2 configuration. This is a summary of what I did (I also tested other ports, mounted directories etc.): #docker system prune --all --force #docker run -dit --user=root -p 10401:10401 -v /home/robertwinkler/dataspace/nextcloud/DATA/:/data spctools/tpp apache2ctl -DFOREGROUND f1ad3670f4f7117db97c9cff8061a95df718c748e348660a0ca1c78818a0e848
# docker ps CONTAINER ID IMAGE COMMAND CREATED STATUS PORTS NAMES f1ad3670f4f7 spctools/tpp "apache2ctl -DFORE..." 23 minutes ago Up 23 minutes 0.0.0.0:10401->10401/tcp quizzical_bhabha # docker images REPOSITORY TAG IMAGE ID CREATED SIZE docker.io/spctools/tpp latest 73727a87cff0 8 days ago 2.86 GB Best, Robert On Dec 3 2018, at 6:29 pm, Eric Deutsch <[email protected]> wrote: > Hi Robert, when you get an error like this: > > > Bind for 0.0.0.0:10401 > (https://link.getmailspring.com/link/[email protected]/0?redirect=http%3A%2F%2F0.0.0.0%3A10401&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t) > failed: port is already allocated > > That means that there is already an Apache service listening on the 10401 > point on the Docker *host* machine. The first thing to consider is there > perhaps already another container running on 10401. Maybe you don’t need to > start another container because it’s already running. Based on the your > `docker ps` it looks like this is your issue. Maybe you don’t need to launch > another TPP? Or maybe you would want to shut down that older container before > launching a new one? > > Or it’s possible that you already have TPP installed and running on the > machine that is the Docker host. If that’s the case, you’ll want to map to a > different port so that you can run both at the same time (which is fine), > like this: > > sudo docker pull spctools/tpp > sudo docker run -dit --user=root -p 10402:10401 -v /tmp/tppdata:/data > spctools/tpp apache2ctl -DFOREGROUND > > Here what I’ve done is bind the local port 10402 to the Apache web server > running in the container. You would then use a web browser to go to: > > http://myhost:10402/tpp/cgi-bin/tpp_gui.pl > > Regards, > Eric > > > From: [email protected] > (https://link.getmailspring.com/link/[email protected]/1?redirect=mailto%3Aspctools-discuss%40googlegroups.com&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t) > <[email protected] > (https://link.getmailspring.com/link/[email protected]/2?redirect=mailto%3Aspctools-discuss%40googlegroups.com&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t)> > On Behalf Of Robert > Sent: Saturday, December 1, 2018 12:22 AM > To: spctools-discuss <[email protected] > (https://link.getmailspring.com/link/[email protected]/3?redirect=mailto%3Aspctools-discuss%40googlegroups.com&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t)> > Subject: Re: [spctools-discuss] Re: Running TPP/Petunia GUI with Docker > > > > Hi, I am getting > > > /usr/bin/docker-current: Error response from daemon: driver failed > programming external connectivity on endpoint adoring_engelbart > (33ba8ed4cceaa86424363b5a9f1635ebf4ebb641c47c24c714f2ee1fe8846663): Bind for > 0.0.0.0:10401 > (https://link.getmailspring.com/link/[email protected]/4?redirect=http%3A%2F%2F0.0.0.0%3A10401&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t) > failed: port is already allocated. > > > > opening the http://localhost:10401/ > > shows the default Apache2 Ubuntu Page > > > > docker ps > CONTAINER ID IMAGE COMMAND CREATED STATUS PORTS NAMES > 799753202ce1 ebf55696681a "apache2ctl -DFORE..." 11 hours ago Up 11 hours > 0.0.0.0:10401->10401/tcp dazzling_mahavira > > > > > > > Am Donnerstag, 29. November 2018 01:32:55 UTC+1 schrieb Eric Deutsch: > > > Hi Robert, thanks for testing. Based on our testing, we now changed the > > calling sequence for starting Petunia to this: > > > > > > docker run -dit --user=root -p 10401:10401 -v /tmp/tppdata:/data > > spctools/tpp apache2ctl -DFOREGROUND > > > > > > as described in the refreshed tutorial: > > http://tools.proteomecenter.org/wiki/index.php?title=Running_the_TPP_docker_image > > > > (https://link.getmailspring.com/link/[email protected]/5?redirect=http%3A%2F%2Ftools.proteomecenter.org%2Fwiki%2Findex.php%3Ftitle%3DRunning_the_TPP_docker_image&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t) > > > > > > Let us know what you think of that! > > > > > > Regarding your precautions question, it seems to depend on how Docker is > > installed on your system. If you have to run `sudo docker` for each > > command, then things are written as root. On one of our computers, the IT > > guys have jailed the user docker runs as as UID 100000, so that’s a little > > different. So I’m not sure the best advice here. In the tutorial we suggest > > do a `chmod 777` that seems to make things work out nicely. On Windows, it > > should all be find if the user has admin privileges. But there still might > > be problems if the user doesn’t have admin privileges? I’m uncertain. I > > haven’t tested that yet. > > > > > > Regards, > > Eric > > > > > > > > From: [email protected] (javascript:) > > <[email protected] (javascript:)> On Behalf Of Robert > > Sent: Tuesday, November 27, 2018 3:17 AM > > To: spctools-discuss <[email protected] (javascript:)> > > Subject: Re: [spctools-discuss] Re: Running TPP/Petunia GUI with Docker > > > > > > > > > > Sure, Eric! Could you please give me the recommended command line > > parameters for running TPP Petunia on both systems (Linux and Windows 10)? > > Any precautions concerning the ownership etc. of the host system? > > > > > > > > Am Donnerstag, 22. November 2018 19:47:25 UTC+1 schrieb Eric Deutsch: > > > Hi Robert, great, thanks for the testing! Can you elaborate a little on > > > what the symptoms are here for the file access through the GUI? I think > > > the file access worked fine in my testing. Maybe one potential fix is > > > setting some more permissive privileges in the directory you’re mapping > > > into the --user root container? > > > > > > Run TPP image > > > Start TPP image: > > > docker run -dit --user=root -p 10401:10401 -v > > > /home/robertwinkler/dataspace/nextcloud/DATA/UPS48_yeast_centroided/:/data > > > spctools/tpp > > > > > > Open GUI with Firefox at http://localhost:10401/tpp/cgi-bin/tpp_gui.pl > > > GUI and guest:guest login works! > > > However: Problems with accessing the directory structure/ uploading files. > > > > > > > > > > > > > > > > > > From: [email protected] > > > (https://link.getmailspring.com/link/[email protected]/6?redirect=mailto%3Aspctools...%40googlegroups.com&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t) > > > <[email protected] > > > (https://link.getmailspring.com/link/[email protected]/7?redirect=mailto%3Aspctools...%40googlegroups.com&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t)> > > > On Behalf Of Robert > > > Sent: Thursday, November 22, 2018 7:11 AM > > > To: spctools-discuss <[email protected] > > > (https://link.getmailspring.com/link/[email protected]/8?redirect=mailto%3Aspctools...%40googlegroups.com&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t)> > > > Subject: [spctools-discuss] Re: Running TPP/Petunia GUI with Docker > > > > > > > > > > > > The complete Test on Linux 64 bit and Windows 10 > > > TPP 5.2 Docker CLI on Linux > > > System and software > > > > > > Docker version 1.13.1, build accfe55-unsupported > > > > > > Docker image: docker.io/spctools/tpp > > > (https://link.getmailspring.com/link/[email protected]/9?redirect=http%3A%2F%2Fdocker.io%2Fspctools%2Ftpp&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t) > > > ID ebf55696681a > > > > > > running on Fedora 29, Intel® Core™ i7-7700HQ CPU @ 2.80GHz, 16 Gb RAM > > > > > > > > > Data > > > > > > Reference data set from ProteomeXchange repository, identifier PXD001819 > > > (Journal of Proteomics 132 (2016) 51–62), Orbitrap Velos data of human > > > standard proteins (UPS Sigma) spiked into yeast background. > > > > > > Conversion of raw data to .mzML profile, then to .mfg centroid data using > > > docker run -it --privileged=true -v > > > /home/robertwinkler/dataspace/nextcloud/DATA/UPS48_yeast/mzML_profile/:/data > > > chambm/pwiz-skyline-i-agree-to-the-vendor-licenses wine msconvert --mgf > > > /data/*.mzML > > > > > > Target data base ups_human_yeast.fasta, composed of : Uniprot Homo > > > sapiens + Uniprot Saccharomyces cervisiae + Sigma UPS proteins sequences > > > Size of .mgf files: ~700 Mb each, 27 files > > > FASTA with 33,183 entries > > > > > > > > > Start Docker > > > > > > # systemctl start docker > > > > > > 1. Command Line Interface (CLI) > > > Start TPP docker image with mounted data directory: > > > docker run -it --privileged=true -v > > > /home/robertwinkler/dataspace/nextcloud/DATA/UPS48_yeast_centroided/:/data > > > spctools/tpp bash > > > > > > > > > Run comet PSM search > > > > > > go to data directory data/mgf and create comet parameters file: > > > comet -p > > > change name to comet.params and edit > > > database_name = ups_human_yeast.fasta > > > decoy_search = 1 > > > > > > Start comet search: > > > time comet *.mgf > > > time real 28m45.053s, ~1min/sample, ~140 Mb/sample > > > > > > > > > Run PeptideProphet > > > > > > in the directory of comet .pep.xml results run: for i in *.pep.xml; do > > > PeptideProphetParser $i; done > > > ~15 s/sample, ~80 Mb/sample > > > > > > > > > Run ProteinProphet > > > > > > in the directory of PeptideProphet .pep.xml results run: for i in > > > *.pep.xml; do ProteinProphet $i $i.prot.xml NOGROUPS; done > > > ~3 s/sample, ~7 Mb/sample > > > > > > > > > 2. Petunia GUI session (on Linux 64 bit) > > > System and software > > > > > > Docker version 1.13.1, build accfe55-unsupported > > > > > > Docker image: docker.io/spctools/tpp > > > (https://link.getmailspring.com/link/[email protected]/10?redirect=http%3A%2F%2Fdocker.io%2Fspctools%2Ftpp&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t) > > > ID ebf55696681a > > > > > > running on Fedora 29, Intel® Core™ i7-7700HQ CPU @ 2.80GHz, 16 Gb RAM > > > > > > > > > Run TPP image > > > > > > Start TPP image: > > > docker run -dit --user=root -p 10401:10401 -v > > > /home/robertwinkler/dataspace/nextcloud/DATA/UPS48_yeast_centroided/:/data > > > spctools/tpp > > > > > > Open GUI with Firefox at http://localhost:10401/tpp/cgi-bin/tpp_gui.pl > > > GUI and guest:guest login works! > > > However: Problems with accessing the directory structure/ uploading files. > > > > > > > > > TPP 5.2 Docker CLI on Windows > > > System and software > > > > > > Docker version 18.06.1-ce, build e68fc7a > > > > > > Docker image: docker.io/spctools/tpp > > > (https://link.getmailspring.com/link/[email protected]/11?redirect=http%3A%2F%2Fdocker.io%2Fspctools%2Ftpp&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t) > > > ID ebf55696681a > > > > > > running on OS Name Microsoft Windows 10 Enterprise, Processor Intel® > > > Core™ i5-3470S CPU @ 2.90GHz, 2901 Mhz, 4 Core(s), 4 Logical > > > Processor(s), 8 Gb RAM > > > > > > > > > Data > > > > > > MS/MS data from Waters Synapt DDA experiments, exported to .mgf (see > > > below). 2 samples with ~400 Mb each. > > > > > > FASTA database composed from EST database and Uniref50 plants, created by > > > cat db_1.fasta db_2.fasta > uni50plantsplus.fasta, 3,557,556 entries. > > > Data set not public yet (sorry). > > > > > > > > > Convert Waters Synapt data to mgf > > > > > > · load msconvert image with vendor libraries: docker run -it -v > > > C:\TPP\data:/data chambm/pwiz-skyline-i-agree-to-the-vendor-licenses bash > > > > > > · dive into data directory and run with LockMass Filter: > > > wine msconvert --filt "lockmassRefiner mz=785.8426" --mgf *.raw > > > > > > > > > · exit image by exit. > > > · Start TPP docker session: > > > docker run -it -v C:\TPP\data:/data spctools/tpp bash > > > > > > > > > Comet PSM search > > > use comet.params from above, just change database_name = > > > uni50plantsplus.fasta > > > > > > with .mgf files, .fasta sequence database and comet.params in the same > > > directory, run: > > > time comet *.mgf Load spectra killed; change in comet.params: > > > spectrum_batch_size = 1000 > > > > > > > > > Run PeptideProphet > > > in the directory of comet .pep.xml results run: for i in *.pep.xml; do > > > PeptideProphetParser $i; done > > > > > > > > > Run ProteinProphet > > > in the directory of PeptideProphet .pep.xml results run: for i in > > > *.pep.xml; do ProteinProphet $i $i.prot.xml NOGROUPS; done > > > > > > > > > Convert prot.xml to HTML/EXCEL > > > > > > In the directory of .prot.xml data run: > > > for i in *.prot.xml; do /usr/local/tpp/cgi-bin/ProtXMLViewer.pl -file $i > > > -action ExportExcel; done > > > Leave TPP docker session by exit and enjoy your results. > > > > > > > > > Conclusion > > > > > > The TPP docker image ebf55696681a (version 5.2) runs on both, Linux and > > > Windows 10 host, in the CLI mode as expected. > > > > > > > > > > > > > > > > > > Am Freitag, 16. November 2018 16:39:06 UTC+1 schrieb Robert: > > > > TPP 5.2 Docker on Linux > > > > # System and software > > > > - Docker version 1.13.1, build accfe55-unsupported > > > > - Docker image: docker.io/spctools/tpp > > > > (https://link.getmailspring.com/link/[email protected]/12?redirect=http%3A%2F%2Fdocker.io%2Fspctools%2Ftpp&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t) > > > > ID ebf55696681a > > > > - running on Fedora 29, Intel(R) Core(TM) i7-7700HQ CPU @ 2.80GHz, 16 > > > > Gb RAM > > > > > > > > # Data > > > > - Reference **data set** from ProteomeXchange repository, identifier > > > > PXD001819 (Journal of Proteomics 132 (2016) 51–62), Orbitrap Velos data > > > > of human standard proteins (UPS Sigma) spiked into yeast background. > > > > - **Conversion of raw data** to .mzML profile, then to ``.mfg`` > > > > centroid data using > > > > ``docker run -it --privileged=true -v > > > > /home/robertwinkler/dataspace/nextcloud/DATA/UPS48_yeast/mzML_profile/:/data > > > > chambm/pwiz-skyline-i-agree-to-the-vendor-licenses wine msconvert > > > > --mgf /data/*.mzML`` > > > > - **Target data base** ``ups_human_yeast.fasta``, composed of : Uniprot > > > > _Homo sapiens_ + Uniprot _Saccharomyces cervisiae_ + Sigma UPS proteins > > > > sequences > > > > Size of .mgf files: ~700 Mb each, 27 files > > > > FASTA with 33183 entries > > > > > > > > # Start Docker > > > > ``# systemctl start docker`` > > > > > > > > # 1. Command Line Interface (CLI) > > > > - **Start TPP docker image** with **mounted data directory**: > > > > ``docker run -it --privileged=true -v > > > > /home/robertwinkler/dataspace/nextcloud/DATA/UPS48_yeast_centroided/:/data > > > > spctools/tpp bash `` > > > > > > > > ## Run comet PSM search > > > > - go to data directory data/mgf and **create comet parameters file**: > > > > ``comet -p`` > > > > change name to comet.params and edit > > > > ``database_name = ups_human_yeast.fasta > > > > decoy_search = 1`` > > > > - Start comet search: > > > > ``time comet *.mgf`` > > > > time real 28m45.053s, ~1min/sample, ~140 Mb/sample > > > > > > > > ## Run PeptideProphet > > > > - in the directory of comet .pep.xml results run: > > > > ``for i in *.pep.xml; do PeptideProphetParser $i; done`` > > > > ~15 s/sample, ~80 Mb/sample > > > > > > > > ## Run ProteinProphet > > > > - in the directory of PeptideProphet .pep.xml results run: > > > > ``for i in *.pep.xml; do ProteinProphet $i $i.prot.xml NOGROUPS; done`` > > > > ~3 s/sample, ~7 Mb/sample > > > > > > > > ## Convert to HTML/EXCEL > > > > ``pepxml2html`` not found. > > > > Leaving TPP docker session by ``exit``. > > > > - Conversion with own script > > > > <https://bitbucket.org/lababi/protyquant/src/06deaeb70a09b8121ce0adc1d7d6da389afe7175/python-scripts/protxml_to_tsv.py?at=master > > > > > > > > (https://link.getmailspring.com/link/[email protected]/13?redirect=https%3A%2F%2Fbitbucket.org%2Flababi%2Fprotyquant%2Fsrc%2F06deaeb70a09b8121ce0adc1d7d6da389afe7175%2Fpython-scripts%2Fprotxml_to_tsv.py%3Fat%3Dmaster&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t)>: > > > > ``for i in *.prot.xml; do python3 protxml_to_tsv.py $i; done`` > > > > > > > > # 2. Petunia GUI session > > > > - **Start TPP image:** > > > > ``docker run -dit --user=root -p 10401:10401 -v > > > > /home/robertwinkler/dataspace/nextcloud/DATA/UPS48_yeast_centroided/:/data > > > > spctools/tpp`` > > > > - **Open GUI** with Firefox at > > > > <http://localhost:10401/tpp/cgi-bin/tpp_gui.pl> > > > > GUI and guest:guest login works! > > > > However: Problems with accessing the directory structure/ uploading > > > > files. > > > > > > > > > > > > Am Montag, 22. Oktober 2018 14:49:30 UTC+2 schrieb Robert: > > > > > Dear TPP friends, > > > > > has anyone tried (or even solved) to run the TPP/Petunia GUI from a > > > > > Docker container/image? > > > > > Best, Robert > > > -- > > > You received this message because you are subscribed to the Google Groups > > > "spctools-discuss" group. > > > To unsubscribe from this group and stop receiving emails from it, send an > > > email to [email protected] > > > (https://link.getmailspring.com/link/[email protected]/14?redirect=mailto%3Aspctools-discu...%40googlegroups.com&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t). > > > To post to this group, send email to [email protected] > > > (https://link.getmailspring.com/link/[email protected]/15?redirect=mailto%3Aspctools...%40googlegroups.com&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t). > > > Visit this group at https://groups.google.com/group/spctools-discuss > > > (https://link.getmailspring.com/link/[email protected]/16?redirect=https%3A%2F%2Fgroups.google.com%2Fgroup%2Fspctools-discuss&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t). > > > For more options, visit https://groups.google.com/d/optout > > > (https://link.getmailspring.com/link/[email protected]/17?redirect=https%3A%2F%2Fgroups.google.com%2Fd%2Foptout&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t). > > > > > > > > > > > > > > > -- > > You received this message because you are subscribed to the Google Groups > > "spctools-discuss" group. > > To unsubscribe from this group and stop receiving emails from it, send an > > email to [email protected] (javascript:). > > To post to this group, send email to [email protected] > > (javascript:). > > Visit this group at https://groups.google.com/group/spctools-discuss > > (https://link.getmailspring.com/link/[email protected]/18?redirect=https%3A%2F%2Fgroups.google.com%2Fgroup%2Fspctools-discuss&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t). > > For more options, visit https://groups.google.com/d/optout > > (https://link.getmailspring.com/link/[email protected]/19?redirect=https%3A%2F%2Fgroups.google.com%2Fd%2Foptout&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t). > > > > > > > > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to [email protected] > (https://link.getmailspring.com/link/[email protected]/20?redirect=mailto%3Aspctools-discuss%2Bunsubscribe%40googlegroups.com&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t). > To post to this group, send email to [email protected] > (https://link.getmailspring.com/link/[email protected]/21?redirect=mailto%3Aspctools-discuss%40googlegroups.com&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t). > Visit this group at https://groups.google.com/group/spctools-discuss > (https://link.getmailspring.com/link/[email protected]/22?redirect=https%3A%2F%2Fgroups.google.com%2Fgroup%2Fspctools-discuss&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t). > For more options, visit https://groups.google.com/d/optout > (https://link.getmailspring.com/link/[email protected]/23?redirect=https%3A%2F%2Fgroups.google.com%2Fd%2Foptout&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t). > > > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to [email protected] > (https://link.getmailspring.com/link/[email protected]/24?redirect=mailto%3Aspctools-discuss%2Bunsubscribe%40googlegroups.com&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t). > To post to this group, send email to [email protected] > (https://link.getmailspring.com/link/[email protected]/25?redirect=mailto%3Aspctools-discuss%40googlegroups.com&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t). > Visit this group at https://groups.google.com/group/spctools-discuss > (https://link.getmailspring.com/link/[email protected]/26?redirect=https%3A%2F%2Fgroups.google.com%2Fgroup%2Fspctools-discuss&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t). > For more options, visit https://groups.google.com/d/optout > (https://link.getmailspring.com/link/[email protected]/27?redirect=https%3A%2F%2Fgroups.google.com%2Fd%2Foptout&recipient=c3BjdG9vbHMtZGlzY3Vzc0Bnb29nbGVncm91cHMuY29t). > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
