Hi, I have acquired the DDA and DIA data on various tissues, and trying to build a spectral library by combining the DDA results of all tissues, that can be used further for the DIA data analysis. I am trying two approaches- one assay library generation with SpectraST and other using skyline to build the library. Now, I confused, how should I proceed.
Should I combine the peptideprophet results of various tissues by using iProphet? Or, create assay libraries for each tissue separately using the SpectraST and then import them separately in a common Skyline document? I would be very grateful for your help. Thanks in advance. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
