Hi David,

Thanks for the suggestion. I also suspected the same and currently doing 
the manual check. However, as I said, Comet did not report any error! My 
other query is, which format should I take as raw data (mzML/mzXML) as 
search input and how much it differs in terms of peptide identified 
(pep.xml)? I also need to combine the result of comet and tandem after that.

Thanks,
Sudip

On Wednesday, July 31, 2019 at 12:00:38 AM UTC+5:30, David Shteynberg wrote:
>
> Hi Sudip,
>
> Based on the message it appears that your fasta database may contain amino 
> acid letter codes that make Tandem fail.   Can you check your fasta 
> database to make certain it contains only valid protein sequences?
>
> Thanks,
> -David
>
> On Tue, Jul 30, 2019 at 8:15 AM sudip ghosh <[email protected] 
> <javascript:>> wrote:
>
>> Hi All,
>>
>> I am trying to search some mzML/mzXML (converted from .wiff) files in X! 
>> Tandem but it fails repeatedly (RETURN CODE:65280, 256). While the same 
>> set of mzML file was searched successfully in Comet. I am attaching the 
>> param file. Please help me out.
>>
>> Regards,
>> Sudip
>>
>> [image: XTandem error.png]
>>
>>
>>
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