I was taking a look at Jake's table of amino acid masses and found some differences for 15N labelled:
Maybe I made some mistake, but I think it is worth to take a look: A 72.03415 R 160.10111 N 116.04293 D 116.02694 C 161.0307 E 130.04259 Q 130.05858 G 58.02146 H 140.05891 I 114.08406 L 114.08406 K 130.09496 M 132.04049 F 148.06841 P 98.05276 S 88.03203 T 102.04768 W 188.07931 Y 164.06333 V 100.06841 All the best, Carlo On Friday, November 21, 2008 at 12:31:53 AM UTC, Jake W wrote: > > Ah. Thanks for that. Still seem to have trouble, though. So when I > run > > run_in c:/Inetpub/wwwroot/ISB/data/ASAPRa > tioTesting20Nov08; xinteract - > N240Min_50ppm_err_ASAP1.interact.pep.xml -p0 -l6 > -x20 -OAp -A-lACDEFGHIKLMNPQRSTVWY-r0.05- > mA72.03415R160.10111N118.04293D117.0269 > 4C105.00919E130.04259Q130.05858G59.02146H140.05891I116.08406L114.08406K130.09496 > > > M133.04049F148.06841P98.05276S88.03203T102.04768W187.07931Y165.06333V101.06841 > > > c > :/Inetpub/wwwroot/ISB/data/ > ASAPRatioTesting20Nov08/240Min_50ppm_err.tandem.pep.x > ml > > I get: (I'll just put the ASAPRatio Parser output here, everything > else seems OK): > > running: "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser > "240Min_50ppm_err_ASAP1.inte > ract.pep.xml" -lACDEFGHIKLMNPQRSTVWY -r0.05 - > mA72.03415R160.10111N118.04293D117. > 02694C105.00919E130.04259Q130.05858G59.02146H140.05891I116.08406L114.08406K130.0 > > > 9496M133.04049F148.06841P98.05276S88.03203T102.04768W187.07931Y165.06333V101.068 > > > 41" > adjusting 143.004 to 143.059 > label A: 0 vs 0 > label D: 0 vs 0 > label F: 0 vs 0 > label G: 0 vs 0 > label H: 0 vs 0 > label I: 0 vs 0 > label K: 0 vs 0 > label L: 0 vs 0 > label N: 0 vs 0 > label P: 0 vs 0 > label R: 0 vs 0 > label S: 0 vs 0 > label T: 0 vs 0 > label V: 0 vs 0 > label W: 0 vs 0 > label Y: 0 vs 0 > adjusting 143.004 to 143.059 > (same repeated ~15 times) > adjusting 143.004 to 143.059 > WARNING: Trying to compute mass of non-residue: r > WARNING: Trying to compute mass of non-residue: o > WARNING: Trying to compute mass of non-residue: t > WARNING: Trying to compute mass of non-residue: e > WARNING: Trying to compute mass of non-residue: i > adjusting 143.004 to 143.059 > (same repeated ~20 times) > adjusting 143.004 to 143.059 > command completed in 200 sec > > running: "C:/Inetpub/tpp-bin/ASAPRatioProteinRatioParser > "240Min_50ppm_err_ASAP1 > .interact.prot.xml"" > command completed in 297 sec > > running: "C:/Inetpub/tpp-bin/ASAPRatioPvalueParser > "240Min_50ppm_err_ASAP1.inter > act.prot.xml"" > no pvalues available for this dataset > command completed in 1 sec > > Any further ideas? > > many thanks, > Jake > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/6e0a138a-e89d-4f8b-b74f-cfc3ecc205ac%40googlegroups.com.
