It has made 48 .PARAMS files and 1 .TANDEM file.

 tandem_params.xml is intact.

On Monday, 28 September 2020 at 15:41:49 UTC+5:30 [email protected] wrote:

> Hello,
>
> I am a novice using  TPP v5.2.0 Flammagenitus on  a workstation (Intel, 
> Xeon, E5-2630 ver.5, 32Gb RAM, win 10). I have successfully run Comet, 
> however, the X!Tandem search is still on command 1 out of 48. Its been more 
> than 56 hours. How much time does it take to complete the search? I am 
> working on 48.Raw files from a Thermo QExactive. The size of each file is 
> approx. 500Mb. 
>
> Fixed Mod: Carbamidomethyl on cysteine,   Var mod : Oxidation of 
> methionine and N-terminal acetylation.
>
> What is the issue with the search? 
>
>  
> *The params file is: *
> *<?xml version="1.0" encoding="UTF-8"?>*
>
> *-<bioml>*
>
> *<note label="list path, default parameters" 
> type="input">D:/TPP/data/params/isb_default_input_kscore.xml</note>*
>
> *<note label="spectrum, path" type="input">full_mzXML_filepath</note>*
>
> *<note label="output, path" type="input">full_tandem_output_path</note>*
>
> *<note label="output, log path" type="input"/>*
>
> *<note label="output, sequence path" type="input"/>*
>
> *<note label="list path, taxonomy information" 
> type="input">D:/TPP/data/params/taxonomy.xml</note>*
>
> *<note label="protein, taxon" type="input">protein_database</note>*
>
> *<note label="spectrum, parent monoisotopic mass error minus" 
> type="input">2.0</note>*
>
> *<note label="spectrum, parent monoisotopic mass error plus" 
> type="input">4.0</note>*
>
> *<note label="spectrum, parent monoisotopic mass error units" 
> type="input">Daltons</note>*
>
> *<note label="spectrum, parent monoisotopic mass isotope error" 
> type="input">no</note>*
>
> *<note label="residue, modification mass" type="input">57.021464@C</note>*
>
> *<note label="residue, potential modification mass" 
> type="input">15.994915@M</note>*
>
> *<note label="residue, potential modification motif" type="input"/>*
>
> *<note label="protein, N-terminal residue modification mass" 
> type="input"/>*
>
> *<note label="protein, C-terminal residue modification mass" 
> type="input"/>*
>
> *<note label="protein, cleavage semi" type="input">yes</note>*
>
> *<note label="scoring, maximum missed cleavage sites" 
> type="input">2</note>*
>
> *<note label="refine" type="input">no</note>*
>
> *<note label="refine, maximum valid expectation value" 
> type="input">0.1</note>*
>
> *<note label="refine, modification mass" type="input">57.012@C</note>*
>
> *<note label="refine, potential modification mass" 
> type="input">15.994915@M</note>*
>
> *<note label="refine, potential modification motif" type="input"/>*
>
> *<note label="refine, cleavage semi" type="input">yes</note>*
>
> *<note label="refine, unanticipated cleavage" type="input">no</note>*
>
> *<note label="refine, potential N-terminus modifications" type="input"/>*
>
> *<note label="refine, potential C-terminus modifications" type="input"/>*
>
> *<note label="refine, point mutations" type="input">no</note>*
>
> *<note label="refine, use potential modifications for full refinement" 
> type="input">no</note>*
>
> *</bioml>*
>

-- 
You received this message because you are subscribed to the Google Groups 
"spctools-discuss" group.
To unsubscribe from this group and stop receiving emails from it, send an email 
to [email protected].
To view this discussion on the web visit 
https://groups.google.com/d/msgid/spctools-discuss/f9f29898-4464-4f88-8bd5-4a1be0018986n%40googlegroups.com.

Reply via email to