Hi Steven, what do your comet parameters look like? I'm not sure if this is the problem, but for files with many scans it is good to set the `spectrum_batch_size` parameter to something reasonable, like 10000. The instructions are found in the "fragment ions" comments section of the default parameters file.
On Friday, September 17, 2021 at 1:58:10 AM UTC-4 [email protected] wrote: > Hi, > > I'm a fairly new user to TPP and this sort of analysis in general and had > a question about input file sizes during Comet Search. > I have downloaded some MS data from a publication via an online database > in .mzXML format (the .raw files weren't provided) and have been trying to > run a peptide database search using the Comet software with the default > param settings. However I consistently get the message "Warning - no > spectra searched" and no output file is created (screenshot attached). The > mzXML file itself is actually quite large (~26GB) and I was wondering if > the size of the input file itself might be the issue? In the case where the > input files would be quite large, is there a potential workaround? > > Apololgies if I've left out any critical information here > > Thanks in advance, > Steven > > [image: TPP error.png] > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/ccbb39b3-f5f8-4702-a0dd-587633ac7b2an%40googlegroups.com.
