Hello again Malcolm,

There are several of these non-ascii characters in the protXML file that
you sent.  After I removed them I was able to open the file in
ProtXMLViewer.  Also the syntax errors from tpp2mzid went away (although I
wasn't able to convert your file without the associated pepXML data.)
 Please let us know if you are able to resolve this issue on your end.

Cheers,
-David

On Fri, Jun 10, 2022 at 7:35 AM Malcolm Cook <[email protected]> wrote:

> I just got TPP installed and am trying to [test the installation with a
> sample dataset](
> http://tools.proteomecenter.org/wiki/index.php?title=TPP_6.1.0:_Installing_on_Ubuntu_20.04_LTS#Testing_the_installation_with_a_sample_dataset_.28optional.29
> )
>
> I am getting an error with tpp2mzid parsing ProteinProphet output (here
> is the full file
> <https://ftp.stowers.org/public/mec/tpp/interact.prot.xml>).
>
> Reviewing the ISB/SPC Trans Proteomic Pipeline :: Jobs logs I read our
> ProteinProphet completed with success:
>
>
> *Finished. Results written to:
> /data/tpp/tests/QuickYeastUPS1/interact.prot.xmlhowever *
> however trying to process it with tpp2mzid gives error
>
>
>
>
> *Reading:
> /data/tpp/tests/QuickYeastUPS1/interact.prot.xml/data/tpp/tests/QuickYeastUPS1/interact.prot.xml(148)
> : error 4: Syntax error parsing XML.Failed to read protXML:
> /data/tpp/tests/QuickYeastUPS1/interact.prot.xmlmanually *
> inspecting this file it looks like a well formed big ball of XML to my
> eyes:
>
>
>
>
>
>
>
>
>
>
>
>
>
>
> *<?xml version="1.0" encoding="UTF-8"?><?xml-stylesheet type="text/xsl"
> href="http://hd1991356yb/tests/QuickYeastUPS1/interact.prot.xsl
> <http://hd1991356yb/tests/QuickYeastUPS1/interact.prot.xsl>"?><protein_summary
> xmlns="http://regis-web.systemsbiology.net/protXML
> <http://regis-web.systemsbiology.net/protXML>"
> xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance
> <http://www.w3.org/2001/XMLSchema-instance>"
> xsi:schemaLocation="http://sashimi.sourceforge.net/schema_revision/protXML/protXML_v9.xsd
> <http://sashimi.sourceforge.net/schema_revision/protXML/protXML_v9.xsd>"
> summary_xml="/data/tpp/tests/QuickYeastUPS1/interact.prot.xml"><protein_summary_header
> reference_database="/data/tpp/tests/QuickYeastUPS1/Yeast_UPS_cRAP.fasta"
> residue_substitution_list="I -> L"
> source_files="/data/tpp/tests/QuickYeastUPS1/interact.ipro.pep.xml"
> source_files_alt="/data/tpp/tests/QuickYeastUPS1/interact.ipro.pep.xml"
> min_peptide_probability="0.20" min_peptide_weight="0.50"
> num_predicted_correct_prots="98.6" num_input_1_spectra="0"
> num_input_2_spectra="348" num_input_3_spectra="70" num_input_4_spectra="0"
> num_input_5_spectra="0" initial_min_peptide_prob="0.05"
> total_no_spectrum_ids="357.6" sample_enzyme="trypsin"><program_details
> analysis="proteinprophet" time="2022-06-09T09:15:57" version="
> Insilicos_LabKey_C++ (TPP v6.1.0 Parhelion, Build 202206081159-exported
> (Linux-x86_64))"><proteinprophet_details occam_flag="Y" groups_flag="Y"
> degen_flag="Y" nsp_flag="Y" fpkm_flag="N" initial_peptide_wt_iters="2"
> nsp_distribution_iters="3" final_peptide_wt_iters="0"
> run_options="IPROPHET"> <nsp_information
> neighboring_bin_smoothing="Y"><yadda_yadda_yadda>...</yadda_yadda_yadda></protein_group></protein_summary>*
> trying to run  tpp2mzid  from the command line sheds little light:
>
>
>
>
>
>
>
>
> */usr/local/tpp/bin/tpp2mzid
> /data/tpp/tests/QuickYeastUPS1/interact.prot.xmltpp2mzid v0.9.11 (May 19
> 2022), copyright Mike Hoopmann, Institute for Systems Biology.Built using
> mzIMLTools: 1.2.6    Mar 21 2022Built using NeoPepXMLParser: 1.0.2    2022
> MAY 17Built using NeoProtXMLParser: 1.0.0    2022 MAY 19Reading:
> /data/tpp/tests/QuickYeastUPS1/interact.prot.xml/data/tpp/tests/QuickYeastUPS1/interact.prot.xml(148)
> : error 4: Syntax error parsing XML.Failed to read protXML:
> /data/tpp/tests/QuickYeastUPS1/interact.prot.xml*
> how to diagnose/debug/fix?
>
> Thanks!
>
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