Hi Charu, Thank you very much for your reply! I am sorry for such a long delay in responding, but I was out of my office.
I am a little bit confused – does DIALib-QC support spectral libraries from Skyline? I tried to assess several ion libraries via web-interface at https://db.systemsbiology.net/sbeams/cgi/PeptideAtlas/AssessDIALibrary, both in Skyline format (non-redundant .blib file) as well as in Spectronaut format export from Skyline (.csv and .txt). Unfortunately, I was not successful as after analysis – no data were shown. I have not tried a desktop version yet. My libraries have different sizes, but non-redundant libraries are below 200 MB (typically between 10 and 120 MB), redundant libraries are over 100 MB up to 500 MB. So I hope that it should not be the issue in case of non-redundant library. Thank you very much, I would appreciate the possibility to send you a demo version of one of my libraries. Should I share it here or directly or should I use a sharing service (e.g. WeTransfer)? Thank you once again! Best wishes, Jakub Dne pátek 21. října 2022 v 18:10:22 UTC+2 uživatel [email protected] napsal: > Hi Jakub, > > Thankyou for using DIALib-QC for assessing your skyline library. For > libraries more than 200MB, you need to download > <http://www.swathatlas.org/DIALibQC.php> and install the DIALibQC tool > locally to your machine for its assessment. What is your library size? > Also can you please share a demo tiny version of your skyline library for > me to look at? > > Best, > Charu > > > Charu K Midha, PhD, > > Post Doc Fellow > > Moritz's Lab, ISB, > > > > > On Fri, Oct 21, 2022 at 7:28 AM Jakub Červenka <[email protected]> > wrote: > >> Dear all, >> >> I would like to use your DIALib-QC tool for spectral libraries that >> were built in Skyline. I tried to export library from Skyline in format for >> Spectronaut (Skyline Schema for exporting Spectronaut compatible libraries >> - https://biognosys.com/resources/spectronaut-further-user-resources/) >> and used this file for DIALib-QC tool ( >> http://www.swathatlas.org/DIALibQC.php), but I was not able to get any >> results. In fact, have got only an empty web page (see attachment). >> >> Is there any way how to analyze spectral libraries from Skyline? I >> contacted Skyline support for help, but we were not successful with running >> DIALib-QC tool ( >> https://skyline.ms/announcements/home/support/thread.view?rowId=57092). >> >> Would be possible to add the Skyline type of spectral libraries to >> supported formats for DIALib-QC tool? I am sure that many Skyline users >> would appreciate it. >> >> Thank you very much! >> >> >> >> Jakub >> >> >> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to [email protected]. >> To view this discussion on the web visit >> https://groups.google.com/d/msgid/spctools-discuss/a432c2a7-43a8-45f0-bd78-d7d7a8bfbb69n%40googlegroups.com >> >> <https://groups.google.com/d/msgid/spctools-discuss/a432c2a7-43a8-45f0-bd78-d7d7a8bfbb69n%40googlegroups.com?utm_medium=email&utm_source=footer> >> . >> > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/15508608-36d6-4a3c-a689-6078c8a463a3n%40googlegroups.com.
