Oh ! thank you Let me explain- I have mixed two samples light and heavy labelled proteins (at cell level) But if the cell count was not equal in the two groups, it will result in unequal contribution of proteins to the mixture. In this case the ratio L/H for individual protein will not be correct. The solution is normalization (as we do in St. Peter)
so I wanted to know if such normalization is done in Express before calculating the light to heavy ratio. On Wed, Nov 22, 2023 at 3:07 PM Jimmy Eng <[email protected]> wrote: > I don't know what "normalized intensity/peak area" of each peptide means > in this context but I can say that XPRESS doesn't do any normalization. It > simply reports light area to heavy area ratios. You'll have to do > adjustments after the fact i.e. centering the mean ratio to 1:1 if you > expect the bulk of your samples to be 1:1. > On Wednesday, November 22, 2023 at 1:12:35 PM UTC-8 sudarshan kumar wrote: > >> How do we know that the express ratio is calculated from the normalized >> intensity /peak area of each peptide? >> >> I ask this question because when we mix the light and heavy samples, >> there may be difference in the initial quantity of protein taken from the >> two groups. >> > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to [email protected]. > To view this discussion on the web visit > https://groups.google.com/d/msgid/spctools-discuss/ed5c84e0-054a-4070-a058-3a3237b5bf45n%40googlegroups.com > <https://groups.google.com/d/msgid/spctools-discuss/ed5c84e0-054a-4070-a058-3a3237b5bf45n%40googlegroups.com?utm_medium=email&utm_source=footer> > . > -- ------------------------------------------------------------------- The real voyage of discovery consists not in seeking new lands but seeing with new eyes. — Marcel Proust Dr. Sudarshan Kumar (Fulbright-Nehru Fellow) (B.V.Sc.& A.H., M.V.Sc., PhD.) Sr. Scientist Animal Biotechnology Center (Proteomics and Cell Biology Lab.) National Dairy Research Institute Karnal, 132001 Haryana, India Contact No 09254912456 URL www.ndri.res.in Orcid Id: https://orcid.org/0000-0002-9816-4307 -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/CALZrgHTvTEzKHR2DRRc6%2Bu9RT%2BDgPc5Qxa%2BQDQO81Pfgyb0R0Q%40mail.gmail.com.
