Hi all,

I notice another small issue (maybe this is my win11 problem?): when 
running Kojak within TPP, I set the threads to 24 or 28. However, in the 
task manager, I notice the main search was always running on exact 
CPU16-31, which seem to be E-cores of this 13900K. Did anyone notice 
similar problem?   

[image: Snipaste_2024-05-28_22-14-14.png]
[image: Snipaste_2024-05-28_22-15-57.png]

Best,
Zeyu 





On Monday, May 27, 2024 at 11:12:09 AM UTC-4 Zeyu Wang wrote:

> Hi David,
>
> Thanks! After replacing the current kojak.exe, It seems that now the kojak 
> searching can continue without error message.
>
> Best,
> Zeyu 
>
> On Friday, May 24, 2024 at 9:55:03 PM UTC-4 David Shteynberg wrote:
>
>> Hello again,
>>
>> After a bit of digging I found that this is likely caused by a bug in the 
>> current TPP compiled and distributed version of Kojak that will have to be 
>> corrected for the next release.  Meanwhile you should be able to download 
>> and use the official Kojak build available here:  
>> https://kojak-ms.systemsbiology.net/download.html
>>
>>
>> This version will require additional .net dependecies!
>>
>> Please replace the current kojak executable in c:/TPP/bin with the 
>> executable from the zip file and try running kojak again.
>>
>>
>> Best of luck and keep us posted to your progress.
>>
>> -David
>>
>> On Fri, May 24, 2024, 1:07 PM Zeyu Wang <[email protected]> wrote:
>>
>>> Hi David,
>>>
>>> Thank you! Here are the two kojak parameter files I tested (the original 
>>> and the customized).  Link: Kojak_Troubleshooting 
>>> <https://gtvault-my.sharepoint.com/:f:/g/personal/zwang3136_gatech_edu/EnxqUYYUZGNDvCEG8kB-vtsB3Q2GfNvcqaAygJ_0ttBEFA?e=Ub1nub>
>>>  .
>>>
>>> Best,
>>> Zeyu 
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>> On Friday, May 24, 2024 at 3:47:38 PM UTC-4 David Shteynberg wrote:
>>>
>>> Hello Zeyu,
>>>
>>> Thanks for your interest in TPP and Kojak.  Yes we maintain and use 
>>> Kojak in the latest release.  To help us troubleshoot this problem, would 
>>> you mind sharing the parameter file used in your test?
>>>
>>> Thank you!
>>> -David
>>>
>>> On Fri, May 24, 2024, 12:28 AM Zeyu Wang <[email protected]> wrote:
>>>
>>> Hi,
>>>
>>> Are there anyone using Kojak in the TPP v7.0.0 ?  Today I follow the 
>>> instruction and try to run a quick test. But at the very beginning, it 
>>> reports an error. 
>>>
>>> "Kojak version 2.1.0, October 16 2023 Copyright Michael Hoopmann, 
>>> Institute for Systems Biology Visit http://kojak-ms.org for full 
>>> documentation. ****** Begin Kojak Analysis ****** Time at start: Fri May 24 
>>> 02:52:02 2024 Parameter file: c:/TPP/data/params/Kojak_2.conf 12 
>>> preconfigured crosslinkers. Reading FASTA database: 
>>> c:/TPP/data/dbase/UP000005640_9606_20230222.fasta
>>> ...
>>>   Total Proteins: 20594
>>>   Adding Kojak-generated decoys. New Total Proteins: 41188
>>>   5658462 peptides to search (2785870 linkable).
>>>
>>> Fri May 24 02:52:45 2024 ERROR: 
>>> ERROR: cannot open \c:\TPP\data\Test_Kojak\E_ZW_LPS_XL_F10.kojak.txt for 
>>> output. Please ensure path and write permissions are correct."
>>>
>>> Is the error due to the output file path format? Or shall I adjust the 
>>> read/write permission of the TPP?
>>>
>>> Best,
>>> Zeyu
>>>
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