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Article Title:
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Mold Testing Methods Surface Samples

Article Description:
====================

When a mold inspector takes bulk, tape, carpet/dust, or swab
samples, spore numbers are not compared quantitatively to outdoor
levels. Thus, the number of spores in these types of samples
often are not as meaningful as the numbers found in air samples.


Additional Article Information:
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565 Words; formatted to 65 Characters per Line
Distribution Date and Time: 2007-06-07 11:00:00

Written By:     Daryl Watters
Copyright:      2007
Contact Email:  mailto:[EMAIL PROTECTED]



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Mold Testing Methods Surface Samples
Copyright (c) 2007 Daryl Watters
A Accredited Mold Inspection Service, Inc.
http://www.floridamoldinspectors.us



When a mold inspector takes bulk, tape, carpet/dust, or swab
samples, spore numbers are not compared quantitatively to outdoor
levels. Thus, the number of spores in these types of samples
often are not as meaningful as the numbers found in air samples.
Also, because the air is not being tested, your inspector cannot
say for sure how much if any of the mold sampled from surfaces is
in the air you are breathing. However, these samples can be
helpful because they typically provide the analyst with more than
just the mold spores so that identification of mold type can be
more accurately conducted by viewing various structures of the
mold, not just spores. In addition to providing more structure
for direct microscopic examination, bulk samples are sometimes
grown in the lab or run through PCR testing for analysis to the
species level.

Mold Testing with Tape

When a tape sample of actual mold from a moldy surface is taken
using Biotope, a clear piece of Scotch Tape, or a sticky Cyclex
slide, the sample will often show entire mold structures
including spore forming structures and hyphea. These can be used
to confirm mold growth more confidently and rule out the
possibility that the sample was just settled spores only.

Mold Testing of Bulk Samples

When a bulk sample of actual mold or moldy material is sent to a
lab, the lab may use clear Scotch Tape to take a sample from the
bulk mold material for examination under the microscope. The lab
may culture some of the bulk mold specimen in a Petri dish for
analysis of the colonies to the species level.

Mold Testing of Carpet Dust

When a dust sample is analyzed it may be place on a slide for
direct examination to view spores hidden in the dust directly.
This is a very common method used by most mold inspectors labs
and has become accepted in the industry. This popular method may
be helpful but many spores are not seen because spores are hidden
behind dust, or the spores blend in well with dust. The lab will
often report very low spore levels even if the carpet sampled was
obviously very moldy. This inspector has seen this happen many
times with various dust samples tested at different labs. When
studies are done on what are normal and what are elevated spore
levels in carpet dust, the scientist working on the projects and
the mold labs they utilize for dust analysis use very different
methods for analysis.

They wash the dust and dust filter out of the collector with a
mild solvent and culture the spores in a petri dish. This method
will typically reveal tens of thousands of spores or even
hundreds of thousands or millions of spores. Your inspector must
be aware of the different methods and the different results to be
expected when interpreting dust sample results.

Mold Testing with Swabs

A sterile swab provided by a microbiology lab is sometimes used
for sampling. This inspector dislikes this method because unlike
when using tape, the mold structures are always broken up when
using swabs. Therefore, meaningful mold structure identification
and spore counting cannot be done when testing mold with swabs.
Many poorly trained mold inspectors will use a swab on nearly
every inspection done, not because of a well thought out sampling
plan, but simply because the lab gave them swabs.




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Daryl Watters has a bachelors degree in education for teaching 
biology and general science and is a certified mold inspector, 
certified home inspector, and certified indoor environmentalist 
providing building inspections in South Florida since 1993. For 
more information visit http://www.floridamoldinspectors.us  
http://www.florida-mold-inspection.com




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