Based on a quick search on Serial EM CCD, I suggest you check with folks that provide the software at the University of Colorado, Boulder. https://bio3d.colorado.edu/SerialEM/

Can you read the original data files with the latest version of their software? Can you export from the data files to TIFF or any other format using the latest version of their software? Have you followed their error reporting procedure?

I am of course assuming this is the software that you are using since you did not specify that in the email to the list.

Richard Nolde
    1. StropOffsets error on some TIFFs (Bourque, Cole)
    2. Re: StropOffsets error on some TIFFs (Olivier Paquet)

----------------------------------------------------------------------

Message: 1
Date: Mon, 21 Mar 2022 15:10:43 +0000
From: "Bourque, Cole" <[email protected]>
To: "[email protected]" <[email protected]>
Subject: [Tiff] StropOffsets error on some TIFFs
Message-ID: <[email protected]>
Content-Type: text/plain; charset="us-ascii"

Hi all,

I have a collection of TIFFs from some microscope imaging, but several of them 
can't be opened or otherwise manipulated, showing instead this error when I 
investigate them using LibTIFF in MobaXterm:

MissingRequired: TIFF directory is missing required "StripOffsets" field.

These TIFFs were collected during one imaging session with the same program 
(SerialEM), and the StripOffsets error is distributed among the TIFFs seemingly 
randomly. As in 10 are OK, then 50 aren't, then 10 are OK, etc.

With the suggestion of our "IT guy", I am using LibTIFF to try to attempt a repair after he confirmed that the files 
weren't corrupted. It was recommended that I take the StripOffset values from the "working" TIFFs and move that value 
over to the "broken" TIFFs, but I can't figure out how to begin to approach that (if it is possible). I have attached 
some information from a "working" TIFF from the "tiffinfo" command as a txt file. I hope that helps.

To summarize, here is my question:

Is it possible to copy the StripOffset information from the "working" TIFFs to the 
"broken" TIFFs to (hopefully) allow them to be decompressed for viewing?

Best regards,
Cole Bourque


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