Not always, but usually you can get a pretty good idea unless the computer uses an SoC design. Think of it this way: if you have a keyboard connected to a PS/2 port, and you've verified that the keyboard doesn't have any sort of wireless card built into it, you know that it's only sending its data through that port. (I use PS/2 as an example because USB is more complicated, and it's possible to trick the OS into sending data it shouldn't by pretending to be a device that should be getting that data.)

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