This blog post from @Ev in early October says "more than 165m"
http://blog.twitter.com/2010/10/newtwitterceo.html

On Mon, Oct 25, 2010 at 8:23 AM, Slate Smith <sl...@slatesmith.com> wrote:

> Anyone have any idea what the estimated userbase is currently? I've looked
> around for the ~count but closest I see is a march @mashable article saying
> ~200m. Anyone?
>
> - Slate
>
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