Same problem here on my Asus EeePC 1015px on Ubuntu Mate 16.04.1.
After a clean O.S installation the speakers worked for few days, but today I 
found that I got this issue also.
I can confirm that using headphones the sound is properly reproduced.
The operating system has all the updates available to 2016/11/06 installed.

Another strange thing I noticed is that the alert volume bar keeps
returning at lowest position at every reboot, no matter if I pull it up;
please check the attached image.

I also booted a live Ubuntu Mate 16.04.1 (from the usb flash disk I used
to install the O.S few days ago) and I'm still unable to play sound from
speakers.

Here's all entries I found in syslog after boot regarding the audio
subsystem:

Nov  6 14:30:05 ubuntu-mate kernel: [   55.525107] snd_hda_codec_realtek 
hdaudioC0D0: autoconfig for ALC269VB: line_outs=1 (0x14/0x0/0x0/0x0/0x0) 
type:speaker
Nov  6 14:30:05 ubuntu-mate kernel: [   55.525125] snd_hda_codec_realtek 
hdaudioC0D0:    speaker_outs=0 (0x0/0x0/0x0/0x0/0x0)
Nov  6 14:30:05 ubuntu-mate kernel: [   55.525137] snd_hda_codec_realtek 
hdaudioC0D0:    hp_outs=1 (0x1a/0x0/0x0/0x0/0x0)
Nov  6 14:30:05 ubuntu-mate kernel: [   55.525145] snd_hda_codec_realtek 
hdaudioC0D0:    mono: mono_out=0x0
Nov  6 14:30:05 ubuntu-mate kernel: [   55.525154] snd_hda_codec_realtek 
hdaudioC0D0:    inputs:
Nov  6 14:30:05 ubuntu-mate kernel: [   55.525165] snd_hda_codec_realtek 
hdaudioC0D0:      Mic=0x12
Nov  6 14:30:14 ubuntu-mate pulseaudio[3715]: [pulseaudio] authkey.c: Failed to 
open cookie file '/home/ubuntu-mate/.config/pulse/cookie': File o directory non 
esistente
Nov  6 14:30:14 ubuntu-mate pulseaudio[3715]: [pulseaudio] authkey.c: Failed to 
load authentication key '/home/ubuntu-mate/.config/pulse/cookie': File o 
directory non esistente
Nov  6 14:30:14 ubuntu-mate pulseaudio[3715]: [pulseaudio] authkey.c: Failed to 
open cookie file '/home/ubuntu-mate/.pulse-cookie': File o directory non 
esistente
Nov  6 14:30:14 ubuntu-mate pulseaudio[3715]: [pulseaudio] authkey.c: Failed to 
load authentication key '/home/ubuntu-mate/.pulse-cookie': File o directory non 
esistente
Nov  6 14:30:14 ubuntu-mate pulseaudio[3856]: [pulseaudio] pid.c: Daemon 
already running.
Nov  6 14:30:39 ubuntu-mate pulseaudio[3715]: [pulseaudio] bluez5-util.c: 
GetManagedObjects() failed: org.freedesktop.DBus.Error.NoReply: Did not receive 
a reply. Possible causes include: the remote application did not send a reply, 
the message bus security policy blocked the reply, the reply timeout expired, 
or the network connection was broken.

** Attachment added: "Screenshot at 2016-11-06 14_29_36.png"
   
https://bugs.launchpad.net/ubuntu/+source/alsa-driver/+bug/1606078/+attachment/4773469/+files/Screenshot%20at%202016-11-06%2014_29_36.png

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https://bugs.launchpad.net/bugs/1606078

Title:
  No sound with Ubuntu 16.04

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