--- Jeff Brosius <[EMAIL PROTECTED]> wrote:
> To: "Paramedicine List" <[EMAIL PROTECTED]>
> 
> Pursuant to the recent discussion on thrombolytics and CVA.
> 
> Enjoy the reading.
> 
> Best,
> 
> Jeff Brosius
> Paramedic, etc.
> Atlanta, GA
> www.prehospital-perspective.com
> [EMAIL PROTECTED]
> "The fate of the wounded rest
> in the hands of the one that
> applies the first dressing."
> -- Nicholas Senn, 1896
> 
> 
> ----- Original Message -----
> From: "Jeffrey Mann" <[EMAIL PROTECTED]>
> To: <[EMAIL PROTECTED]>
> Sent: Thursday, May 16, 2002 6:39 PM
> Subject: [EMED-L] An open letter to the stroke interventionist
> community regarding tPA for acute ischemic stroke
> 
> 


From: "Jeffrey Mann" <[EMAIL PROTECTED]>
To: <[EMAIL PROTECTED]>
Sent: Thursday, May 16, 2002 6:39 PM
Subject: [EMED-L] An open letter to the stroke interventionist
community
regarding tPA for acute ischemic stroke


The debate about the risk:benefit ratio of tPA therapy in acute
ischemic stroke continues.

 Read the following dialogue articles in the May 2002 issue of the
Western Journal of Medicine.

 1) Truth about the NINDS study: setting the record straight -
Jeffrey Mann

 Available at http://www.ewjm.com/cgi/content/full/176/3/192

 2) Why were the benefits of plasminogen activator (tPA) exaggerated?
- Griffin Trotter

 Available at http://www.ewjm.com/cgi/content/full/176/3/194

 3) Thrombolysis for acute ischemic stroke: still a treatment for the
few by the few - Joanna M Wardlaw, Richard I Lindley, Steff Lewis

 Available at http://www.ewjm.com/cgi/content/full/176/3/198

 The NINDS trial is the only RCT that has shown any benefit from tPA
therapy and many clinicians have questioned the validity of the NINDS
trial for a number of reasons -- that all the other RCTs have failed
to demonstrate the efficacy of tPA, that the NINDS trial's results
could possibly be due to chance [1], that the NINDS trial was far too
small in size, that the NINDS trial's results are not readily
transferable to
 clinical practice because 50% of the patients were treated in < 90
minutes (a level of clinical performance that is unlikely to be
achievable in community practice), and that certain post-marketing
studies have shown higher rates of secondary ICH in community
practice, which may/may not be due to protocol violations. However,
despite these various criticisms, no one has suggested that the NINDS
trial is internally invalid because of methodological or
interpretative flaws.

 My personal analysis of the NINDS trial suggests that the NINDS
trial, as originally reported in the NEJM, is internally invalid.
When I originally wrote my dialogue piece about 9 months ago, I
wondered to what degree the imbalance in baseline stroke severity
between the tPA-treated patients and the placebo patients in the
NINDS trial's 91-180 minutes groups, could account for the favorable
results of the
 NINDS trial -- and I therefore wondered to what degree the NINDS
trial could be perceived to be internally invalid. The answer is
still not entirely clear to me, and I wonder how clear it will be to
the stroke interventionist community after they read my dialogue
piece in the wjm.

 In the original NEJM paper, the NINDS trialists gave no indication
that there was any imbalance in baseline stroke severity between the
tPA-treated and placebo patients. They also did not comment on the
unexpected fact that the odds ratio (OR) for an excellent stroke
outcome was 1.7 (mRS<=1) for the 0-90 minutes groups and 2.4 (mRS<=1)
for the 91-180 minutes groups, which suggested that tPA was more
effective if given later, rather than earlier. The NINDS trialists
were obviously disconcerted by this finding, and they continued to
analyse their data in private (interestingly, although they generated
many hypotheses to account for the unexpected finding, it was
obviously due to the fact that they did not correct for the imbalance
in baseline stroke severity between the treated and placebo
patients). Five years later, the NINDS trialists supplied new figures
for the ORs based on a re-analysis of their raw data using an
unspecified statistical adjustment for baseline NIHSS scores and
other baseline variables - in the article by Marler [2]. However,
they only supplied a revised OR for the global statistic stroke
outcome measure, and they apparently never published revised OR
figures for the most commonly used stroke outcome measure - the
modified Rankin score (mRS<=1). Without knowing the revised OR for a
mRS<=1, how
 can a stroke interventionist compare the revised results to the
original OR for a mRS<=1, or to the excellent stroke outcome results
obtained from other stroke trials, which are usually reported as
mRS<=1? Without understanding the statistical adjustment that the
NINDS trialists used to create their revised OR figures, how can a
stroke interventionist determine whether it adequately corrected for
the confounding variable of imbalances in baseline stroke severity?
According to the TOAST study [3], the graphical curve delineating the
natural relationship between
 baseline stroke severity and the rate of excellent stroke outcome in
untreated stroke patients is very steep, and each one point change in
baseline NIHSS stroke severity score could cause a 5-10% difference
in the absolute rate of an excellent stroke outcome due to the
natural course of the disease. Therefore, any statistical adjustment
that the NINDS statisticians used to correct for imbalances in
baseline stroke severity has to be fully compatible with that fact.
It would be extremely useful if the NINDS statisticians publicise the
methodology of their statistical adjustment, so that other
independent statisticians can judge whether it is compatible with the
TOAST graph.

 In that same Marler article [2], the NINDS trialists plotted the
re-estimated efficacy of tPA against time-to-treatment as a
hypothetical model graph-curve (figure 2) and it can be seen that tPA
has borderline-positive efficacy after 150 minutes, and the lower
boundary of the 95% confidence interval line intersects the "no
benefit for tPA" X axis at 150 minutes (see reference number [4] for
a hand-drawn copy of
 figure 2 - the copy is freely viewable online). That model
graph-curve suggests that tPA has limited (and equivocal) efficacy
after 150 minutes, which would be compatible with Jerome Hoffmann's
observation [5] that "the study showed an overall 11-13% absolute
benefit with tPA treatment; however, a recent report [2] by the NINDS
authors clarified that the benefits were greater than this in the
"very early" (0-90 min) group, which means that they had to be less
than this in the "early" (90-180 min) group". Also, the fact that the
lower boundary of the 95% confidence interval crosses the "no benefit
for tPA" X axis at 150 minutes strongly suggests that the NINDS
trial's sample size was too small. Gordon Guyatt states in JAMA's
Users' Guides to the Medical Literature [6] "In a positive trial
establishing that the effect of
 treatment is greater than zero, look at the lower boundary of the
confidence interval to determine whether the sample size has been
adequate. If this lower boundary - the smallest plausible treatment
effect compatible with the data - is greater than the smallest
difference that you consider important, the sample size is adequate
and the trial is definitive. If the lower boundary is less than this
smallest important difference, the trial is non-definitive and
further trials are required".

 Does the stroke interventionist community fully understand how that
hypothetical model graph was devised, and whether it is statistically
valid and accurate?  Should the NINDS trialists have plotted the
model graph using the OR for the most frequently used favorable
stroke outcome measure - the mRS<=1 (instead of the global
statistic)? What would the revised efficacy figures be if the NINDS
trialists had used relative
 risk measurements rather than odds ratio measurements to quantify
the benefit of tPA therapy - after adjusting for imbalances in
baseline variables?

 Barbara Tilley, the primary statistician for the NINDS trial,
co-wrote in an article [7] that "odds ratios have been used
extensively in data collected both prospectively (for example, a
cohort study) or retrospectively (for example, a case control study)
when logistic regression is used to adjust for covariates. However,
the odds ratio is
 less commonly used to communicate the primary results of a clinical
trial because of its lack of clinical interpretation. It measures
neither a relative size nor an absolute size difference for the
treatment effect on the outcome. As an alternative, the relative risk
(RR), unlike the odds ratio, measures the relative size of the
treatment difference, that is, the ratio of the response probability
in a treatment group versus the response probability in a reference
group."

In that same article, Barbara Tilley re-analysed the NINDS trial's
data using a log link model rather than a logit link model, and found
that the log link model estimated the benefit of tPA to be 1.32
(relative risk estimate) for part II of the NINDS trial while the
logit link model had previously estimated the benefit of tPA to be
1.73 (odds ratio estimate). In fact, Barbara Tilley wrote further
"Considering the NINDS t-PA stroke trial, if the analysis plan had
specified a goodness-of-link test to choose the link function, we
would have conducted the global test for the treatment effect based
on the log link rather than the logit link. We could then interpret
the results using an estimate of relative risk instead of the odds
ratio, -----". It is refreshing to note that the NINDS statisticians
agree that it is better to use relative risk measurements, rather
than odds ratio measurements, to determine the efficacy of tPA, and
it is interesting to see how much lower the RR figure is than the OR
figure. Although I am very sceptical of the accuracy of the RR figure
of 1.32 (because the NINDS statisticians gave no indication that
major statistical adjustments were needed to correct for imbalances
in baseline stroke severity between the treated and placebo patients)
note that the RR figure of 1.32 was for the entire time span of 0-180
minutes. Can you imagine, after examining the graph in reference
number 4, how low the RR figure must be for patients treated between
91-180 minutes, and particularly between 150-180 minutes? The
relative benefit of tPA for patients treated in the 91-180 time
period may only be a fraction of the benefit experienced by patients
treated in the 0-90 time period (the estimation of relative benefit
is based on the simple awareness that most of the patients treated in
the 0-90 minute time period were treated between 60-90 minutes and
the estimated odds ratio for a favorable stroke outcome is 2.8-3.8
for that time period, while the estimated odds ratio for the 91-180
time period is between 1.3-2.8, and most of those patients were
 actually treated between 150-180 minutes and the estimated odds
ratio was 1.3-1.8 for the 150-180 time period -- note that all of the
estimated odds ratio figures are derived from the Marler graph
(reference number [4]).

 If one cannot precisely determine the adjusted RRR for patients
treated between 91-180 minutes, then one cannot precisely calculate
the absolute risk reduction (risk difference) for those patients.
Barbara Tilley wrote [7] a "risk difference (RD) is the difference in
response probabilities between two groups, which is sometimes
considered clinically more interpretable than the relative risk,
because it indicates an absolute but not relative treatment size
difference in response probability. It is an intuitively appealing
measure of treatment efficacy in clinical trials. The risk difference
is useful in that it provides an estimated amount by which a
particular response might be increased or reduced if a specified
treatment is removed, and is a particularly important concept when
treatment benefits are offset by side-effects and/or by a high cost."
In other words, Barbara Tilley is suggesting that one needs to know
the absolute benefit of tPA therapy (RD) in order to calculate a
risk:benefit ratio for tPA therapy. I agree - but how can a stroke
interventionist determine the risk:benefit ratio of tPA therapy for
stroke patients treated between 91-180 minutes,
 assuming that the absolute risk of a major side-effect (symptomatic
ICH) is approximately 6%, if he does not precisely know the absolute
benefit of tPA therapy for those same patients (after having to make
precise statistical adjustments to correct for imbalances in baseline
stroke severity between treated and placebo patients)? Also, any
absolute benefit figure that is used for the 91-180 minute time span
would only be an "average" figure and one really needs to know the
absolute benefit for different time spans throughout that 90 minute
time period (eg. 90-120 minutes, 120-150 minutes, 150-180 minutes)
because the efficacy of  tPA continuously wanes throughout the 91-180
minute time period. (To better appreciate the varying relationship
between RRR and the NNT with respect to variations in
time-to-treatment and/or variations in baseline stroke severity, see
reference number [8] which is freely available online)

 Should stroke interventionists be obliged to offer the stroke
patient more precise information about the risks and benefit of tPA
therapy - with particular attention paid to natural variations in
baseline stroke severity and "real life" variations in
time-to-treament?

 For instance, what are the answers to the following three questions
with reference to 4 hypothetical stroke patients?

 Patient 1 = baseline NIHSS score of 8.
 Patient 2 = baseline NIHSS score of 12.
 Patient 3 = baseline NIHSS score of 16.
 Patient 4 = baseline NIHSS score of 20.

 Question 1: What is the likelihood of an excellent stroke outcome
(mRS<=1) without any treatment for those 4 patients?

 Question 2: What is the likely benefit of tPA therapy for those 4
patients if they are treated in the following time frames = < 90
minutes, 90-120 minutes, 120-150 minutes and 150-180 minutes?

 Question 3: What is the likely secondary ICH rate for those 4
patients if they are treated in the following time frames = < 90
minutes, 90-120 minutes, 120-150 minutes and 150-180 minutes?

 How do you answer question number 1?

 I believe that the answer to question number 1 has to take the
natural course of the disease into precise account, and it is well
known that patients with mild strokes have a much better rate of
excellent stroke outcome than patients with severe strokes (due to
the natural course of the disease).

 My "best guess" answer to question number 1 is:-

Expected rate of excellent stroke outcome for patient number 1 
(baseline NIHSS score of 8) = 56% 
Expected rate of excellent stroke outcome for patient number 2 
(baseline NIHSS score of 12) = 42% 
Expected rate of excellent stroke outcome for patient number 3 
(baseline NIHSS score of 16) = 23% 
Expected rate of excellent stroke outcome for patient number 4 
(baseline NIHSS score of 20) = 13%

 The "expected" rate of excellent stroke outcome numbers come from
the TOAST graph curve [3]. Do you use different "expected" figures?
>From where do you obtain your "expected" figures?

 I believe that a stroke interventionist should be able to supply a
stroke patient with a numerical figure for the expected rate of
excellent stroke outcome - derived from the evidence-based medical
literature - so that the individual stroke patient can estimate his
likelihood of an excellent stroke recovery (based on the natural
course of the disease) before he tries to establish whether tPA
therapy (or any
 other stroke therapy) can offer him a better rate of recovery. If
the stroke interventionist community does not agree with the
estimated figures from the TOAST graph, then why does it not perform
a prospective study on 10,000 acute ischemic stroke patients
(untreated) and measure their baseline NIHSS stroke severity scores
and their rate of excellent stroke outcome at 3 months? It would then
be possible to draw a TOAST-like graph showing the precise
relationship between the baseline NIHSS score and the rate of
excellent stroke outcome without having to use a logistic regression
equation to draw a "best-fit" graph (because the graph would be
plotted from actual baseline NIHSS scores for each level of baseline
stroke severity from a NIHSS score of 1-25). By plotting that graph,
the stroke interventionist community would have established a "gold
standard" curve that would allow it to determine the
 true benefit of tPA therapy (or any other stroke therapy) - by
comparing the individual treated patient's results to the "gold
standard" graph.

 How do you answer question number 2?

 I believe, that in the absence of reliable results from a RCT that
is perfectly randomized for baseline stroke severity, that one can
only answer question 2 accuratedly if one plots the results of tPA
therapy for different treatment times (<90 minutes, 90-120 minutes,
120-150 minutes, 150-180 minutes) for each level of baseline stroke
severity (from a NIHSS score of 1-25) on top of that "gold standard"
graph, so that an automatic correction is made for imbalances in
baseline stroke
 severity. If the tPA-treated patient's rate of excellent stroke
outcome figure is higher than the untreated patient's figure (for
each baseline NIHSS score), then tPA obviously works, and the degree
of efficacy will be obvious by seeing how much higher the treated
patient's figure is above the untreated patient's "gold standard"
figure - for varying baseline stroke severity scores and varying
times-to-treatment.

 How do you answer question number 3?

 I believe that one can really only answer question 3 accuratedly if
the stroke interventionist community plots the secondary ICH rate
results similarly - by plotting the secondary ICH rate against the
baseline NIHSS score and by plotting 4 sets of curves for the
different treatment times - because it is well-known that the
secondary ICH rate depends on both the baseline stroke severity and
the degree of delay in administering tPA therapy. Kidwell [9] showed
that the secondary ICH rate increased markedly with baseline NIHSS
scores  10, but their published results are of limited value because
the study sample size was too small, because they used IA tPA, and
because they used a subgroup analysis with subgroups that are too
broad (see reference number [8] for a handpainted copy of their
diagram showing the hemorrhagic
 transformation results - it is freely viewable online). I think that
the ICH rate needs to be plotted for each NIHSS score between 1-25
(and not from limited subgroup data, which is too crudely inaccurate)
and for varying treatment times. The result-analysis may show that
patients with severe strokes (NIHSS score  15), who are treated later
(  150 minutes after stroke onset), have much higher secondary ICH
rates and that the risk:benefit ratio of tPA therapy for those
patients is significantly greater than 1.0.

 The stroke interventionist community already has enough pooled data
from a number of stroke trials [10] to be able to plot those curves.

 By using the pooled data to plot those curves, the variable of
imbalances in baseline stroke severity will no longer have to be
considered a confounding variable, and the true risk:benefit ratio of
tPA therapy will immediately become apparent -- by examining the
excellent stroke outcome rate and the secondary ICH rate for each
 baseline NIHSS score for a variety of treatment times (< 90 minutes,
90-120 minutes, 120-150 minutes, 150-180 minutes).

 "Statistics are like a bikini: what they reveal is suggestive, but
what they conceal is vital" - Aaron Levenstein.

 I think that the stroke research community needs to immediately make
the pooled raw data from all the tPA-for-stroke trials publically
available, so that the vital truth about tPA can be revealed.

 The vital truth regarding tPA's efficacy in acute ischemic stroke
cannot be discovered by examining the result-analyses of the major
tPA-for-stroke RCTs (NINDS, ECASS, ECASS II, ATLANTIS) as they were
originally reported in the medical literature, because they did not
address the confounding effect of the most critical prognostic
variable - imbalances in baseline stroke severity between the
tPA-treated and placebo patients. Even more recent post hoc
re-analyses of those trials, such as the re-analysis of the ATLANTIS
trial's < 3 hours patient group [11] and the meta-analysis of the
NINDS, ECASS, ECASS II and ATLANTIS trials [12] make no attempt to
correct for imbalances in stroke severity between the treated and
placebo patients, thereby rendering their conclusions moot.

 I think that if the stroke interventionist community decides that
the NINDS trial was too small in size and too poorly randomized, and
that arbitrary, and inordinately complex, post hoc statistical
adjustments cannot accurately correct for the marked imbalance in
baseline stroke severity in the 91-180 minutes groups -- then the
need to perform a much larger tPA-for-stroke RCT, that is precisely
balanced for critical
 prognostic variables like baseline stroke severity, becomes
imperative.
 The stroke research community may decide to actively debate the
issues that I have discussed, but if genuine "uncertainty" still
persists, then the need to perform a new RCT will likely remain --
because it makes no sense to base an entire "brain attack" industry
on the results of a single clinical trial, if its legitimacy remains
forever controversial and doubtful. In the interim period, the stroke
interventionist
 community could debate what to do with the presently accumulated raw
data, and it could also debate the advisability, and correct
methodology, of analysing the pooled raw data from multiple RCTs and
observational studies (as I have just described).

 Finally, I think that stroke researchers, who deem themselves
tPA-experts, should actively engage tPA-contrarians and tPA-sceptics
in "open" scientific debate regarding the utility of tPA in acute
ischemic stroke. The tPA-experts should specifically avoid adopting
an "all-knowing" attitude as exemplified by the statement made by
James Grotta in his editorial in the February 2002 issue of the
Stroke
 Interventionalist publication [13], when he wrote "The data
supporting the efficacy of thrombolysis within the first 3 hours
after stroke onset in patients who qualify, as nicely reviewed by Dr
Pancioli, is now unanimously endorsed by those who know best - ie.
those Neurologists and Emergency Physicians who have made the
comittment to take care of acute stroke patients for a living and are
experienced in using
 thrombolytics." The implication of that statement seems to be that
other neurologists and emergency physicians, who do not actively
participate in stroke research, are incapable of accurately analysing
the evidence-based medical literature, and they should remain
passively dependent on the subjective judgments of "those who know
best". The stroke experts are obviously at the cutting edge of stroke
research, and they are the people most keenly aware of potentially
productive research endeavours in the area of biological and
mechanical thrombolysis.
 However, I don't agree that stroke experts are automatically more
adept at judiciously assessing the "weight" of the published
tPA-for-stroke medical literature, and that they will automatically
come to a more rational and better balanced conclusion. It is my
personal belief that any conscientious physician, who has acquired a
modicum of evidence-based medicine skills, can painstakingly review
the tPA-for-stroke medical literature and come to independent
judgments, that may be closer to the vital truth than the biased
judgments of some of the self-appointed tPA-experts. History, of
course, will be the final arbiter, and the future will determine who
is closer to realizing the vital truth about tPA therapy in acute
ischemic stroke -- the tPA-experts, who have a fixed set of a priori
biases, and who are therefore absolutely convinced that they know
best, or the tPA-sceptics who are likely to remain more open-minded
about the issues.

 Jeff Mann.

 References:

 1. Carl E Counsell, Mike J Clarke, Jim Slattery, Peter A G
Sandercock.
 The miracle of DICE therapy for acute stroke: fact or fictional
product
 of subgroup analysis? BMJ 1994;309:1677-1681 (24 December).
Available
 online at http://bmj.com/cgi/content/full/309/6970/1677

 2. Marler, J R. MD. Tilley, B. C. PhD. Lu, M. PhD. Brott, T.G. MD.
 Lyden, P. C. MD. Grotta, J. C. MD. Boderick, J. P. MD. Levine, S. R.
MD.
 Frankel, M.P. MD. Horowitz, S. H. MD. Haley, E. C. Jr. MD.
Lewandowski,
 C. A. Kwiatkowski, T. P. MD. for the NINDS rt-PA Stroke Study Group
*.
 Early Stroke Treatment Associated With Better Stroke Outcome: The
NINDS
 rt-PA Stroke Study. Neurology 55 (11) 1649 - 1655, December 12,
2000.

 3. Adams, H. P. Jr. MD. Davis, P. H. MD. Leira, E. C. MD. Chang,
K.-C.
 MD. Bendixen, B. H. PhD, MD. Clarke, W. R. PhD. Woolson, R. F. PhD.
 Hansen, M. D. MS. Baseline NIH Stroke Scale score strongly predicts
 outcome after stroke: A report of the Trial of Org 10172 in Acute
Stroke
 Treatment (TOAST). Neurology. 53(1):126-131, July 13, 1999

 4. Representative copy of figure 2 from the Marler article.
 Available online at
 http://emguidemaps.homestead.com/files/marlergraph.html

 5. Hoffmann. J.R. Tissue Plasminogen Activator for Acute Ischemic
 Stroke: Is the CAEP position statement too negative? CJEM vol 3
number 3
 July 2001.
 Available at

http://www.caep.ca/004.cjem-jcmu/004-00.cjem/vol-3.2001/v33-183.htm#main

 6. Users' Guides to the Medical Literature: A Manual for
Evidence-based
 Clinical Practice - page 348 - edited by Gordon Guyatt, Drummond
Rennie.

 7. Lu M, Tilley B. Use of odds ratio or relative risk to measure a
 treatment effect in clinical trials with multiple correlated binary
 outcomes: data from the NINDS trial.  Statistics in Medicine 2001;
20:
 1891-1901.

 8. Relationship between baseline risk of a poor stroke outcome and
the
 RRR and the NNT
 Available online at
http://emguidemaps.homestead.com/files/nnttpa.html

 9. Kidwell, Chelsea S. MD; Saver, Jeffrey L. MD; Carneado, Joaquin
MD;
 Sayre, James PhD; Starkman, Sidney MD; et al. Predictors of
Hemorrhagic
 Transformation in Patients Receiving Intra-Arterial Thrombolysis.
 Stroke. 33(3):717-724, March 2002.

 10. Saver JL, Kidwell CS, Starkman S. Commentary: Thrombolysis in
 stroke: it works! BMJ 2002;324:723-729 (23 March).

 11. Albers, Gregory W. MD. Clark, Wayne M. MD. Madden, Kenneth P.
MD,
 PhD. Hamilton, Scott A. PhD. ATLANTIS Trial: Results for Patients
 Treated Within 3 Hours of Stroke Onset. Stroke. 33(2):493-496,
February
 2002.

 12. Fisher, Marc MD; Ringleb, P. A. MD; Schellinger, P. D. MD;
 Schranz,C. MD; Hacke, W. PhD, MD Thrombolytic Therapy Within 3 to 6
 Hours After Onset of Ischemic Stroke: Useful or Harmful? Stroke.
 33(5):1437-1441, May 2002.

 13. The Stroke Interventionalist publication - February 2002 issue.
 Available at http://www.thestrokegroup.com/tsi/pdfs/TSI_24.pdf



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