To whom it may concern:

We are having a problem with the 3' UTR setting of the BioMart Central Portal 
interface. It feels like a bug in the underlying database, so we are hoping you 
would be able to diagnose/fix it.

We need to retrieve the 3' UTRs of yeast genes. I have tried to do this in a 
couple of ways:

TRIAL 1:

DATABASE: Ensembl
Datasets: S cerevisiae genes
Sequences: 3' UTR
Upstream Flank: (blank)
Downstream Flank (blank)
Filters: (default)
Header: Ensembl Gene ID and Associated Gene Name

The result I got was: “Sequence unavailable” for all genes

Then I attempted TRIAL 2:

DATABASE: Ensembl
Datasets: S cerevisiae genes
Sequences: Flank-coding region (not ideal, though, as this is expected to yield 
longer sequences than those we're looking for)
Upstream Flank: (blank)
Downstream Flank: 100 (this is also a problem because although the median 
length of yeast 3' UTRs is 104 bp, they can be as long as ~1,000 bp, so we 
would be missing sequences)
Filters: (default)
Header: Ensembl Gene ID and Associated Gene Name

This works, but with the above caveats.

Thanks in advance for your help.

Sincerely,

Claudio Joazeiro, Ph.D.
Assistant Professor
Department of Cell Biology
The Scripps Research Institute
CB-163
10550 N Torrey Pines Rd
La Jolla, CA  92037
 
Phone: (858) 784-7570
Fax: (858) 784-9779
 




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