Hi Rhoda,

Thanks for your reply and the help from ensembl's regulation team.

I would just like to restate a point to confirm it:

In the *Regulatory evidence query:* Can i expect to get *Raw experimental
annotation* of DNA interaction region with different CellTypes and Assays. *Eg.
ENCODE data.* This information would be cleaned up experimentally, eg
removing noise, but not* filtered by the process of building regulatory
features*.

Cheers
Tomithy



On Wed, Mar 28, 2012 at 11:16 PM, Rhoda Kinsella <[email protected]> wrote:

> Hi Tomithy
> I have contacted a member our Regulation team and he says:
>
> This is essentially correct.  Almost all of the TFs present in the
> evidence will have been used to build the regulatory features (some may
> have been omited as we have some filters based on peak length). The
> situation is slightly different for the histone evidence, there will be a
> higher proportion not represented by a regulatory feature, as they are only
> incorporated when they overlap a TF or site of open chromatin
> (DNase1/FAIRE).
>
> I hope that helps, but if you have further questions please don't hesitate
> to ask
> Regards
> Rhoda
>
>
> On 27 Mar 2012, at 16:07, Arek Kasprzyk wrote:
>
> Hi Tomithy,
> i cc'ing Ensembl experts to help you with your question
>
> a
>
> On Tue, Mar 27, 2012 at 1:47 AM, Tomithy Too <[email protected]>wrote:
>
>> Hi Biomart Admin,
>>
>> I am currently using the biomart services to obtain transcription factor
>> information for my project. I would like to know the difference between the
>> information that I obtain were I to fillup the regulatory features vs
>> regulatory evidence portion of the query form (
>> http://www.ensembl.org/biomart/martview/16aff253720865d2384b56b61a3f1816with 
>> db=Ensembl Regulation 66 for Homo sapiens). This is what I have
>> gathered thus far:
>>
>> *Regulatory features: *A set of consolidated and computed regulatory
>> regions supported by different raw experimental data. It's available for
>> each different cell line.
>> *Regulatory evidence:* Raw experimental annotation of DNA interaction
>> region with different CellTypes and Assays. *Eg. ENCODE data.*
>>
>> I have read through the documentation at the following url but would like
>> to confirm this.
>>
>> http://www.ensembl.org/info/docs/funcgen/index.html
>> http://www.ensembl.org/info/docs/funcgen/regulatory_build.html
>>
>> Many thanks
>> Tomithy
>>
>>
>> _______________________________________________
>> Users mailing list
>> [email protected]
>> https://lists.biomart.org/mailman/listinfo/users
>>
>>
>
>
> --
>
> Arek Kasprzyk, MD, MSc, PhD
> BioMart Project Lead
>
>
> Rhoda Kinsella Ph.D.
> Ensembl Production Project Leader,
> European Bioinformatics Institute (EMBL-EBI),
> Wellcome Trust Genome Campus,
> Hinxton
> Cambridge CB10 1SD,
> UK.
>
>
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